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Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli.
BMC Res Notes
January 2025
Department of Microbiology and Immunology, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt.
Objectives: The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination.
View Article and Find Full Text PDFHydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes.
iScience
December 2024
Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 00000, China.
DNA-modifying enzymes are crucial in biological processes and have significant clinical implications. Traditional quantification methods often overlook enzymatic activity, the true determinants of enzymes' functions. We present hydrogel Bead-based Isothermal Detection (BEAD-ID), utilizing uniform hydrogel bead-based microreactors to evaluate DNA-modifying enzyme activity on-bead.
View Article and Find Full Text PDFCloning and Optimization of Intracellular Expression of Human Interferon β-1a in GS115.
Adv Biomed Res
August 2024
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Interferon-beta (IFN-β) is a cytokine with a wide range of biological and pharmaceutical applications, including multiple sclerosis (MS), cancer, some autoimmune disorders, and viral infectious diseases. Thus, many studies have been performed to develop novel strategies for the high-yield production of functional IFN-β in a cost-effective approach. Here, we aimed to improve the intracellular expression of IFN-β-1a in .
View Article and Find Full Text PDFA computational approach to developing a multi-epitope vaccine for combating Pseudomonas aeruginosa-induced pneumonia and sepsis.
Brief Bioinform
July 2024
Riceland Healthcare, 538 Broadway Ave, Winnie, TX 77665, United States.
Pseudomonas aeruginosa is a complex nosocomial infectious agent responsible for numerous illnesses, with its growing resistance variations complicating treatment development. Studies have emphasized the importance of virulence factors OprE and OprF in pathogenesis, highlighting their potential as vaccine candidates. In this study, B-cell, MHC-I, and MHC-II epitopes were identified, and molecular linkers were active to join these epitopes with an appropriate adjuvant to construct a vaccine.
View Article and Find Full Text PDFDependence of post-segregational killing mediated by Type II restriction-modification systems on the lifetime of restriction endonuclease effective activity.
mBio
August 2024
Waksman Institute for Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, New Jersey, USA.
Article Synopsis
- Plasmid-borne Type II restriction-modification (RM) systems cause post-segregational killing (PSK) due to the loss of restriction and modification enzymes during cell division, leading to the breakdown of unmethylated DNA.
- A CRISPR interference method was developed to investigate PSK and found that different RM systems have distinct stability and recovery behaviors upon plasmid loss, particularly noting the Esp1396I system's limited duration of activity.
- This research suggests that the dynamics of RM systems and host cell growth rates are crucial for understanding PSK, highlighting the need to consider the lifetimes of system components in modeling these processes.
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