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Cloning and functional characterization of the caffeine oxidase gene CsCDH from Camellia sinensis.
Int J Biol Macromol
January 2025
Key Laboratory of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China; National Engineering Research Center for Utilization of Functional Ingredients from Plants, Hunan Agricultural University, Changsha 410128, Hunan, China; Collaborative Innovation Center for Utilization of Functional Ingredients from Plants, Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China. Electronic address:
Theacrine, a purine alkaloid with pharmacological effects such as calming and anti-depressive activities, is biosynthesized through a key rate-limiting enzyme, caffeine oxidase. Despite its importance, the caffeine oxidase gene (CsCDH) in Camellia sinensis has not been cloned to date. We successfully isolated the full-length CsCDH cDNA, which contains a 501-bp open reading frame (ORF) encoding a 166-amino-acid protein with a calculated molecular weight of 18.
View Article and Find Full Text PDFElectrophysiological characterization of human KCNT1 channel modulators and the therapeutic potential of hydroquinine and tipepidine in KCNT1 mutation-associated epilepsy mouse model.
Acta Pharmacol Sin
January 2025
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, 221004, China.
Patients suffering epilepsy caused by the gain-of-function mutants of the hKCNT1 potassium channels are drug refractory. In this study, we cloned a novel human KCNT1B channel isoform using the brain cDNA library and conducted patch-clamp and molecular docking analyses to characterize the pharmacological properties of the hKCNT1B channel using thirteen drugs. Among cinchona alkaloids, we found that hydroquinine exerted the strongest blocking effect on the hKCNT1B channel, especially the F313L mutant.
View Article and Find Full Text PDFGenomic, Evolutionary, and Pathogenic Characterization of a New in Traditional Chinese Medicine .
Viruses
January 2025
Research Center for Life Sciences Computing, Zhejiang Lab, Hangzhou 311100, China.
, a medicinal herbaceous plant documented in the Chinese Pharmacopoeia, is a promising candidate for research into plant-derived pharmaceuticals. However, the study of newly emerging viruses that threaten the cultivation of remains limited. In this study, plants exhibiting symptoms such as leaf yellowing, mottled leaves, and vein chlorosis were collected and subjected to RNA sequencing to identify potential viral pathogens.
View Article and Find Full Text PDFHGS Promotes Tumor Growth, Whereas the Coiled-Coil Domain and Its Oligopeptide of HGS Suppress It.
Int J Mol Sci
January 2025
Biomembrane Group, Tokyo Metropolitan Institute of Medical Science, 6-1-2, Kamikitazawa, Setagaya-Ku, Tokyo 113-8613, Japan.
We previously isolated a cDNA clone for galactosylceramide expression factor 1, which is the rat homologue of hepatocyte-growth-factor-regulated tyrosine kinase substrate (HGS) and induces galactosylceramide expression and morphological changes in COS-7 cells, and reported that overexpression of HGS induced morphological changes in canine kidney epithelial MDCK cells. HGS is a component of the endosomal sorting complexes required for transport machinery that mediates endosomal multivesicle body formation. In this study, the overexpression of HGS induced epithelial-mesenchymal transition and caused transformation in MDCK cells, whereas the overexpression of a coiled-coil domain of HGS inhibited induction of epithelial-mesenchymal transition by HGF stimulation.
View Article and Find Full Text PDFConstruction of Shoot Apical Meristem cDNA Yeast Library of L. and Screening of Proteins That Interact with the Inflorescence Regulatory Factors BnTFL1s.
Curr Issues Mol Biol
December 2024
Academy of Agricultural and Forestry Sciences of Qinghai University, Xining 810016, China.
The determinate inflorescence trait of L. is associated with various desirable agricultural characteristics. ( and ), which encode the transcription factor have previously been identified as candidate genes controlling this trait through map-based cloning.
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