The possibility of congener production in blood during storage was studied. The material consisted of 216 blood specimens in which ethanol was not detected. We divided the specimens into two groups, A and B. Group A: 97 specimens were stored at 4 degrees C until alcohol analysis, and only exposed shortly to room temperature during the aseptic preparation for analysis. Group B: 119 specimens were stored at 17 degrees C until alcohol analysis were performed, and the handling during preparation was not aseptic. All specimens were subsequently stored at 4 degrees C. After three to twelve months of storage the blood specimens were analyzed for congeners. None of the forensic important congeners (n-propanol, isobutanol, or butanol-2) were detected in Group A, whereas in Group B n-propanol was detected in fourteen of the specimens, isobutanol in sixteen, and butanol-2 in two specimens.
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Film-coupled plasmonic resonators offer efficient platforms for light enhancement due to the excitation of gap surface plasmons (GSPs) at metal-insulator-metal interfaces, where electromagnetic energy is stored within the spacer. In applications like biosensing and spontaneous emission control, spatial overlap between the target molecule and plasmonic hotspots is essential. Here, we propose utilizing the controllable, efficient light enhancement capabilities of a specifically designed GSP disk resonator for biosensing and spontaneous emission enhancement.
View Article and Find Full Text PDFPhysiol Rev
January 2025
Metabolism, Obesity, and Nutrition Lab, School of Health, Concordia University, Montréal, Québec, Canada.
Adult males and females have markedly different body composition, energy expenditure, and have different degrees of risk for metabolic diseases. A major aspect of metabolic regulation involves the appropriate storage and disposal of glucose and fatty acids. The use of sophisticated calorimetry, tracer, and imaging techniques have provided insight into the complex metabolism of these substrates showing that the regulation of these processes varies tremendously throughout the day, from the overnight fasting condition to meal ingestion, to the effects of physical activity.
View Article and Find Full Text PDFGenes (Basel)
January 2025
Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia.
As the field of ancient DNA research continues to evolve and produce significant discoveries, it is important to address the crucial limitations it still faces. Under conducive conditions, DNA can persist for thousands of years within human skeletal remains, but, as excavation occurs, the environment abruptly changes, often leading to the loss of DNA and valuable genetic information. Proper storage procedures are needed to mediate DNA degradation and maintain sample integrity.
View Article and Find Full Text PDFVaccines (Basel)
January 2025
Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD 20993, USA.
Background/objectives: Conventional live oral poliovirus vaccines (OPVs) effectively prevent poliomyelitis. These vaccines are derived from three attenuated Sabin strains of poliovirus, which can revert within the first week of replication to a neurovirulent phenotype, leading to sporadic cases of vaccine-associated paralytic poliomyelitis (VAPP) among vaccinees and their contacts. A novel OPV2 vaccine (nOPV2) with enhanced genetic stability was developed recently; type 1 and type 3 nOPV strains were engineered using the nOPV2 genome as a backbone by replacing the capsid precursor polyprotein (P1) with that of Sabin strains type 1 and type 3, respectively.
View Article and Find Full Text PDFArch Public Health
January 2025
School of Nursing and Rehabilitation, Nantong University, Nantong, Jiangsu, 226001, China.
Background: Chinese cancer survivors are not doing well in returning to work. Peer support, as an external coping resource to help cancer survivors return to work, brings together members of the lay community with similar stressors or problems for mutual support. Peer volunteers have not received systematic training, so inappropriate language in the support process can often cause secondary damage to both the peer and the cancer survivor.
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