AI Article Synopsis
- The study examined the replication of two specific plasmids from different bacterial species, Brevibacterium linens and Rhodococcus rhodochrous, in a strain of Rhodococcus sp. R312.
- Researchers developed recombinant plasmids, named pSP33 and pSPC1, which are derivatives of the original plasmids and are suitable for creating new host-vector systems in Rhodococcus sp. R312.
- Both recombinant plasmids contain a gene that provides resistance to neomycin, known as aphI, which is derived from the Tn903 transposon.
Article Abstract
The replication of two cryptic plasmids from Brevibacterium linens ATCC 9174 (pBL33) and Rhodococcus rhodochrous ATCC 4276 (pRC1) was investigated in Rhodococcus sp. R312 (formerly Brevibacterium sp. R312). The recombinant plasmids pSP33 (pBL33 derivative) and pSPC1 (pRC1 derivative) were found to be suitable for establishing new host-vector systems for Rhodococcus sp. R312. They all carry the Tn903 neomycin-resistance-encoding gene (aphI).
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| http://dx.doi.org/10.1016/0378-1119(94)00886-w | DOI Listing |
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