84 suspected patients with gonococcal urethritis were diagnosed by polymerase chain reaction (PCR). It was demonstrated that 28 patients (33.3%) were positive, and 56 (66.7%) negative. Of the PCR-positive patients, 18 showed secretions from the urethral tract, on which the bacterial stain and culture were carried out. The results indicate that 12 patients were positive, and 6 negative. There was no patient with positive stain and culture with PCR-negative. We conclude that PCR is more specific and sensitive than other routine methods in the diagnosis of gonococcal urethritis, especially of chronic infection.
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J Appl Oral Sci
January 2025
Ningde Hospital Affiliated to Ningde Normal University, Department of Stomatology, Fujian, China.
Objective: This study aimed to investigate the role of transmembrane emp24 domain-containing protein 2 (TMED2) in oral squamous cell carcinoma (OSCC).
Methodology: A bioinformatics analysis was first conducted to explore TMED2 expression in OSCC and its relation with overall survival. The analysis results were further verified by assessing TMED2 expression levels in human normal oral keratinocyte cells and human OSCC cell lines using quantitative real-time polymerase chain reaction and the Western blot.
J Cancer Res Clin Oncol
January 2025
Department of Pathology, Theodor Bilharz Research Institute, Giza, 12411, Egypt.
Introduction: Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis. The roles of the transcription factor special AT-rich binding protein-2 (SATB2) and β-catenin in PDAC have been a subject of controversy. We aimed to assess the diagnostic and prognostic impact of SATB2 and β-catenin in PDAC.
View Article and Find Full Text PDFJ Cell Mol Med
February 2025
Department of Reproductive Health and Infertility, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China.
Reduced trophoblast migration and invasion contribute to unexplained recurrent spontaneous abortion (URSA). Aquaporin 3 (AQP3) plays a crucial role in facilitating trophoblast migration and invasion during early pregnancy through fetal-maternal crosstalk. This study aimed to comprehensively investigate the mechanism involving AQP3 and its modulatory effects on human extravillous trophoblast (HTR-8/SVneo cells) migration and invasion.
View Article and Find Full Text PDFInt J Gynecol Cancer
January 2025
All India Institute of Medical Sciences, Department of Obstetrics and Gynecology (Gynecologic Oncology), Rishikesh, Uttarakhand, India. Electronic address:
Objective: To isolate and quantify cell-free DNA, analysis for p53 mutations, and correlation with tumor burden in women with epithelial ovarian cancer compared with benign and borderline epithelial ovarian tumors.
Methods: In this case-control study, plasma samples of eligible women collected 1 hour before surgery and based on final histopathology, women with epithelial ovarian cancer recruited as cases and borderline, and benign ovarian tumors as controls. Cell-free DNA extracted from plasma serum and quantified using Nanodrop Spectrophotometer.
Mikrobiyol Bul
January 2025
Sağlık Bilimleri Üniversitesi, Kayseri Şehir Eğitim ve Araştırma Hastanesi, Parazitoloji Laboratuvarı, Kayseri.
Sıtma, her yıl dünya nüfusunun yarısından fazlası için ciddi bir tehdit oluşturmaya devam etmektedir. Hastalığa neden olan Plasmodium parazitleri, yalnızca insanlarla sınırlı kalmayıp sürüngenlerden kuşlara, memelilerden diğer omurgalılara dek geniş enfeksiyon yelpazesine sahiptir. Plasmodium türleri, çevredeki değişikliklere uyum sağlamalarını sağlayan olağanüstü genetik esnekliğe sahiptir ve bu da onlara sıtma ilaçları gibi tedavi edici maddelere karşı hızla direnç geliştirme ve konakçı özgüllüğünü değiştirme potansiyeli verir.
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