AI Article Synopsis
- The study investigated how factors in the plasma of rats with one-kidney, one-clip hypertension can promote the growth of smooth muscle cells from rat arteries.
- Researchers found that plasma-derived serum from hypertensive rats significantly increased cell growth compared to serum from normotensive rats, particularly when combined with fetal calf serum.
- The study concluded that these humoral factors are stable under freezing and proteolytic treatment but lose their effect when boiled, indicating their unique properties and dependence on certain growth conditions.
Article Abstract
Humoral factors in one-kidney, one-clip (1K,1C) hypertension in rats increase growth of smooth muscle cells cultured from rat arteries. To further characterize the plasma factor or factors involved, we prepared male rats with early, benign 1K,1C hypertension and paired them with one-kidney (1K) normotensive controls. In the presence of growth stimulated by background levels (1%) of fetal calf serum (FCS), plasma-derived serum (PDS), fresh or frozen, from 99 1K,1C rats differentially increased [3H]thymidine incorporation of growth-arrested rat aortic cells; increases were up to 93% more than those evoked by the paired 1K PDS and were concentration related (P < 0.01). However, there was no evidence for a differential effect of 1K,1C PDS in the absence of FCS nor of PDS after boiling. On the other hand, neither treatment of PDS with proteolytic enzymes nor charcoal absorption ablated this differential trophic effect. Thus this study provides evidence that the humoral factor(s) in hypertension are resistant to freezing, proteolysis, and charcoal absorption, but sensitive to boiling, and they require background levels of growth for expression.
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| http://dx.doi.org/10.1152/ajpheart.1995.268.2.H767 | DOI Listing |
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