AI Article Synopsis
- Researchers produced chicken B cell hybridomas that secrete monoclonal antibodies (mAbs) targeting specific antigens found in the sporozoites of Eimeria acervulina.
- The hybridomas were created by fusing infected chicken peripheral blood lymphocytes with a deficient B cell line, and were selected using ELISA to find those that produced mAbs against the sporozoite antigens.
- Two stable hybridomas were identified, P11 and P66, each with distinct binding properties – P11 specifically targeting sporozoite antigens and P66 recognizing a cross-reactive antigen, demonstrating the potential for developing coccidia-specific mAb for research and possibly vaccine production.
Article Abstract
Chicken-chicken B cell hybridomas that secrete monoclonal antibodies (mAb) detecting antigens located at the anterior tip of the sporozoites of Eimeria acervulina have been produced. Peripheral blood lymphocytes (PBL) obtained from chickens infected with E. acervulina were fused with a thymidine-kinase deficient (TK-) chicken B cell line. Hybridomas secreting mAb binding to the sporozoite antigens of E. acervulina were selected using an ELISA. Two stable hybridomas, whose mAb were designated as P11 and P66, respectively, were obtained and their binding characteristics were assessed. The mAb P11 detects the sporozoite but not the merozoite antigens of E. acervulina and Eimeria tenella. The P66 mAb recognizes a cross-reactive antigen of sporozoite and merozoite of E. acervulina but not E. tenella. Flow cytometric analysis of the hybridomas secreting the P11 and P66 mAb showed expression of surface IgM and IgG, respectively. Western blot analysis under reducing conditions showed that mAb P11 recognized a sporozoite protein of 43 kDa. Both mAb recognized antigens located at the anterior end of the sporozoites by indirect immunofluorescence staining. This study shows the development of chicken B cell hybridomas that secrete coccidia-specific mAb.
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| http://dx.doi.org/10.3382/ps.0731685 | DOI Listing |
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