Previous assays for cytidine 3', 5'-cyclic monophosphate (cyclic CMP) have been criticized as being ambiguous. Here a modified RIA protocol, in which the production of assay components has been optimized and a novel trilayer chromatography column separation introduced which successfully separates cyclic CMP from compounds, endogenous to living tissues, which cross-react with anti-cyclic CMP sera, is described. The assay is capable of assaying cyclic CMP between 0.1 and 5 pmol, can be increased in sensitivity by means of an additional acetylation step, and enables the separation of cyclic CMP, cyclic AMP and cyclic GMP so that all three can be estimated in a single sample.
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http://dx.doi.org/10.1080/15321819408009581 | DOI Listing |
Int J Biol Macromol
January 2025
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan. Electronic address:
Radiation-resistant bacteria are of great application potential in various fields, including bioindustry and bioremediation of radioactive waste. However, how radiation-resistant bacteria combat against invading phages is seldom addressed. Here, we present a series of crystal structures of a sensor and an effector of the cyclic oligonucleotide-based anti-phage signaling system (CBASS) from a radioresistant bacterium Deinococcus wulumuqiensis.
View Article and Find Full Text PDFMicrob Cell
July 2024
Department of Biology, University of Copenhagen Copenhagen, DK-2200 Denmark.
We recently characterized the competitive inhibition of cyclic AMP (cAMP) on three periplasmic acid phosphatases, AphA, NadN, and eP4 (Hel), in Rd KW20. This inhibitory effect is vital for orchestrating the nutritional growth and competence development in KW20. Initially discovered in , the function of AphA remains however obscure.
View Article and Find Full Text PDFTalanta
September 2024
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Vanillin finds widespread applications in various industries, such as food, pharmaceuticals, and cosmetics. However, excessive intake of vanillin could pose risks to human health. This study detailed the successful creation of a heterojunction of branched benzopyrazine-based polymers coating on graphene (CMP-rGO) through the Sonogashira-Hagihara coupling reaction.
View Article and Find Full Text PDFInt J Biol Macromol
May 2024
Anhui Provincial Engineering Center for High Performance Biabasd Nylon, College of Materials and Chemistry, Anhui Agricultural University, Hefei, Anhui 230036, China; Biomass Molecular Engineering Center, College of Materials and Chemistry, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address:
Sustainable strategies to improve the water resistance of cellulose paper are actively sought. In this work, polymeric microspheres (PMs), prepared through emulsion polymerization of cellulose nanofibers stabilized rubber seed oil-derived monomer, were investigated as coatings on corrugated medium paper (CMP). After infiltrating porous paper with PMs, the water-resistant corrugated papers (WRCP) with enhanced mechanical properties were obtained.
View Article and Find Full Text PDFFront Neurosci
March 2024
Vanda Pharmaceuticals Inc., Pennsylvania, Washington, DC, United States.
Hyperpolarization-activated cyclic nucleotide-gated (HCN) ion channels generate electrical rhythmicity in various tissues although primarily heart, retina and brain. The HCN channel blocker compound, Ivabradine (Corlanor), is approved by the US Food and Drug Administration (FDA) as a medication to lower heart rate by blocking hyperpolarization activated inward current in the sinoatrial node. In addition, a growing body of evidence suggests a role for HCN channels in regulation of sleep/wake behavior.
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