AI Article Synopsis
- Copolymers of vinyl bases combined with acrylic acid or maleic acid can enhance polyphenylalanine synthesis in a lab setting using E. coli extracts.
- Some of these copolymers, like poly(styrene-maleic acid), can inhibit ribonuclease connected to ribosomes.
- The stimulation effect of specific polymers stems from their ability to disrupt inhibitory proteins that hinder protein synthesis.
Article Abstract
Copolymers of vinyl bases with acrylic acid and styrene or 1-vinyluracil with maleic acid were found to stimulate in vitro polyphenylalanine synthesis using a system extracted from Escherichia coli MRE600. Poly(styrene-maleic acid) was found to inhibit a ribosomal bound ribonuclease. Poly(1-vinyluracil, maleic acid), poly(1-vinyluracil, acrylic acid), and poly(9-vinyladenine, acrylic acid) were not inhibitors of the ribosome bound ribonuclease. The potent (up to fivefold) stimulation by these three polymers is due to the action of the polymers to interfere with ribosomal bound inhibitory protein. A protein, removed by washing ribosomes with 1 M ammonium chloride, characterized by M.J. Miller, A. Niveleau, and A.J. Wahba ((1974) J. Biol. Chem. 249, 3803) has been described as a potent inhibitor of in vitro poly(U)-coded protein synthesis using extracts of Escherichia coli MRE 600.
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| http://dx.doi.org/10.1021/bi00661a022 | DOI Listing |
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