Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1042/bst022332s | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Plant roots affect free-living diazotroph communities in temperate grassland soils despite decades of fertilization.
Commun Biol
July 2024
Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria.
Fixation of atmospheric N by free-living diazotrophs accounts for an important proportion of nitrogen naturally introduced to temperate grasslands. The effect of plants or fertilization on the general microbial community has been extensively studied, yet an understanding of the potential combinatorial effects on the community structure and activity of free-living diazotrophs is lacking. In this study we provide a multilevel assessment of the single and interactive effects of different long-term fertilization treatments, plant species and vicinity to roots on the free-living diazotroph community in relation to the general microbial community in grassland soils.
View Article and Find Full Text PDFImprovement of the nitrogenase activity in Escherichia coli that expresses the nitrogen fixation-related genes from Azotobacter vinelandii.
Biochem Biophys Res Commun
October 2024
Agro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. Electronic address:
The transfer of nitrogen fixation (nif) genes from diazotrophs to non-diazotrophic hosts is of increasing interest for engineering biological nitrogen fixation. A recombinant Escherichia coli strain expressing Azotobacter vinelandii 18 nif genes (nifHDKBUSVQENXYWZMF, iscA, and nafU) were previously constructed and showed nitrogenase activity. In the present study, we constructed several E.
View Article and Find Full Text PDFArchitecture of the RNF1 complex that drives biological nitrogen fixation.
Nat Chem Biol
August 2024
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Freiburg, Germany.
Biological nitrogen fixation requires substantial metabolic energy in form of ATP as well as low-potential electrons that must derive from central metabolism. During aerobic growth, the free-living soil diazotroph Azotobacter vinelandii transfers electrons from the key metabolite NADH to the low-potential ferredoxin FdxA that serves as a direct electron donor to the dinitrogenase reductases. This process is mediated by the RNF complex that exploits the proton motive force over the cytoplasmic membrane to lower the midpoint potential of the transferred electron.
View Article and Find Full Text PDFProteome profiling of Paenibacillus sonchi genomovar Riograndensis SBR5 under conventional and alternative nitrogen fixation.
J Proteomics
March 2024
Departamento de Genética and Programa de Pós-graduação em Genética e Biologia Molecular, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500 - Prédio 43312, Porto Alegre, RS, Brazil. Electronic address:
Paenibacillus sonchi SBR5 is a Gram-positive, endospore-forming facultative aerobic diazotrophic bacterium that can fix nitrogen via an alternative Fe-only nitrogenase (AnfHDGK). In several bacteria, this alternative system is expressed under molybdenum (Mo)-limiting conditions when the conventional Mo-dependent nitrogenase (NifHDK) production is impaired. The regulatory mechanisms, metabolic processes, and cellular functions of N fixation by alternative and/or conventional systems are poorly understood in the Paenibacillus genus.
View Article and Find Full Text PDFGlacial meltwater and seasonality influence community composition of diazotrophs in Arctic coastal and open waters.
FEMS Microbiol Ecol
July 2023
Department of Biology, University of Copenhagen, Strandpromenaden 5, DK-3000 Helsingør, Denmark.
The Arctic Ocean is particularly affected by climate change with unknown consequences for primary productivity. Diazotrophs-prokaryotes capable of converting atmospheric nitrogen to ammonia-have been detected in the often nitrogen-limited Arctic Ocean but distribution and community composition dynamics are largely unknown. We performed amplicon sequencing of the diazotroph marker gene nifH from glacial rivers, coastal, and open ocean regions and identified regionally distinct Arctic communities.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!