AI Article Synopsis
- The basic Ames test is a key method to distinguish between mutagens and non-mutagens but can produce false positives with protein-based substances due to growth factors like histidine.
- The 'suspension variant' of the Ames test was proposed to mitigate these false positives by using rich growth conditions to overpower any growth-promoting substances in the test.
- Key modifications to this new test included plating bacteria that had regrown overnight without histidine and nutrient broth, suggesting a promising direction for refining the Ames test specifically for proteinaceous materials.
Article Abstract
The basic Salmonella/microsome assay (Ames test) is a valuable primary tool by which to discriminate mutagens from non-mutagens. For a variety of chemical test substances this test is easily conducted according to international guidelines for genotoxicity testing. However, the testing of proteinaceous substances in the basic Ames test may generate false positives owing to the presence of growth-promoting constituents in the test sample, such as histidine or its precursors. It was hypothesized that the growth-promoting capacities of biological test samples might be overcome by testing according to the 'suspension variant' of the Ames test, which uses very rich growth conditions thereby overwhelming any growth-enhancing constituents present in a biological test sample. This hypothesis appeared to be correct, although several important modifications had to be made to the suspension assay. The most important aspect of this 'new suspension Ames test' appeared to be the plating of overnight regrown bacteria in the poorest way possible (by omitting histidine and nutrient broth from the overlay agar). This study may comprise an initial step in the development of a modified suspension Ames test for testing proteinaceous substances.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0278-6915(94)90132-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enabling Fast AI-Driven Inverse Design of a Multifunctional Nanosurface by Parallel Evolution Strategies.
Nanomaterials (Basel)
December 2024
Department of Civil, Construction, and Environmental Engineering, Iowa State University, Ames, IA 50011, USA.
Multifunctional nanosurfaces receive growing attention due to their versatile properties. Capillary force lithography (CFL) has emerged as a simple and economical method for fabricating these surfaces. In recent works, the authors proposed to leverage the evolution strategies (ES) to modify nanosurface characteristics with CFL to achieve specific functionalities such as frictional, optical, and bactericidal properties.
View Article and Find Full Text PDFEvaluating the Diagnostic Efficacy of Using Pooled Samples for Chronic Wasting Disease Testing and Surveillance.
Pathogens
December 2024
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Disease monitoring informs the opportunities for intervention by natural resource agencies tasked with managing chronic wasting disease (CWD) in wild cervids. However, allocating funds toward testing can reduce those available for education, outreach, and disease reduction. Implementation of more efficient testing strategies can help meet both an expanding need by resource managers and a burgeoning demand from the hunting public in North America.
View Article and Find Full Text PDFReal-time monitoring of small changes in powder blends and ejected tablets in a low-dose formulation with 1 %w/w of active pharmaceutical ingredient using Raman and near-infrared spatially resolved spectroscopy within a tablet press.
Int J Pharm
January 2025
Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ, 08854, USA; Center for Structured Organic Particulate Systems (C-SOPS), Cranbury, NJ, 08512, USA.
This study used Raman and near-infrared (NIR) spectroscopy to monitor small real-time changes in powder blends and tablets in low-dose pharmaceutical formulations. The research aims to enhance process analytical technology (PAT) in pharmaceutical manufacturing, ensuring high-quality and uniform products with applications to produce drugs with narrow therapeutic indices (NTI). The study utilizes Raman and NIR spatially resolved spectroscopy (SRS) techniques to monitor a moderate cohesive material's active pharmaceutical ingredient (API) concentrations during manufacturing.
View Article and Find Full Text PDFRaiding nature's genetic toolbox for UV-C resistance by functional metagenomics.
Sci Rep
January 2025
NASA Ames Research Center, Planetary Systems Branch, Moffett Field, CA, USA.
As we assess the habitability of other worlds, we are limited by being able to only study terrestrial life adapted to terrestrial conditions. The environments found on Earth, though tremendously diverse, do not approach the multitude of potentially habitable environments beyond Earth, and so limited terrestrial adaptive capabilities tell us little about the fundamental biochemical boundaries of life. One approach to this problem is to use experimental laboratory evolution to adapt microbes to these novel environmental conditions.
View Article and Find Full Text PDFDifferences in metabolite genotoxicity test results of rat liver S9 microsomes treated with various microsomal enzyme inducers.
Toxicol Mech Methods
January 2025
Drug Safety Research and Evaluation, Research, Takeda Pharmaceutical Company Limited, Fujisawa, Japan.
The rat S9 microsome fraction is commonly used to assess compound metabolite formation during genotoxicity assessments. However, methods using S9 have not been standardized for genotoxicity studies, and different experimental methods are used at various facilities. Therefore, this study investigated whether the differences between the two experimental conditions (1) S9 inducers, phenobarbital + beta-naphthoflavones vs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!