The effect of the incorporation of linear (perfluoroalkyl)alkanes (CmF2m + 1CnH2n + 1, FmHn) into liposomes made of DMPC or DPPC on the activity of porcine pancreatic phospholipase A2 was investigated. A large decrease in enzyme activity and modifications of the kinetic profile, especially at and above the phospholipid's phase transition temperature, were observed; both depend on the relative lengths of the phospholipid's fatty acid chains and of the Hn segment of the FmHn molecule. With DMPC Hn must have a minimum of 10 carbon atoms to be effective, as in F6H10, F8H10 and F4H12; F8H8 had no significant hydrolysis-rate-reducing effect. With DPPC Hn must have a minimum of 12 carbon atoms, as in F4H12, while F8H8, F6H10 and F8H10 were ineffective. The absence of effect when C10H22 or C16H34 was incorporated establishes that the fluorinated segment, although its length (from C4 to C8) is not crucial, is required to hinder hydrolysis by PLA2, indicating that this segment plays an important role in structuring the liposomal membrane.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0005-2760(94)00153-p | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spontaneous formation of small and ultrasmall unilamellar vesicles in mixtures of drug surfactant and phospholipid: Effect of chemical structure of phospholipid tails on vesicle size.
J Colloid Interface Sci
December 2024
Department of Medicinal Chemistry, Uppsala University, P.O. Box 547, 751 23, Uppsala, Sweden. Electronic address:
We have investigated the effect of length and chemical structure of phospholipid tails on the spontaneous formation of unilamellar liposomal vesicles in binary solute mixtures of cationic drug surfactant and zwitterionic phosphatidylcholine phospholipids. Binary drug surfactant-phospholipid mixtures with four different phospholipids with identical headgroups (two saturated phospholipids 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC, 14:0) and 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, 16:0), and two unsaturated lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, 18:1) and 1,2-Dierucoyl-sn-Glycero-3-Phosphatidylcholine (DEPC, 22:1)) combined with two different tricyclic antidepressant drugs (amitriptyline hydrochloride (AMT) and doxepin hydrochloride (DXP)) have been investigated with small-angle neutron scattering (SANS) and cryo-transmission electron microscopy (cryo-TEM). We observe a conspicuous impact of phospholipid tail structure on both micelle-to-vesicle transition point and vesicle size.
View Article and Find Full Text PDFPhotophysical behavior of meso-N-butylcarbazole-substituted BODIPY in different nano-scale organized media.
Photochem Photobiol Sci
December 2024
Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600036, Tamil Nadu, India.
The present work focuses on the photophysical behavior of meso-N-butylcarbazole-substituted BODIPY (CBZ-BDP) in different organized media towards exploring the possible use of the dye as a molecular sensor and imaging agent. The molecule shows an appreciable change in absorption and emission spectra at 75% water-acetonitrile mixture compared to pure acetonitrile. In water-acetonitrile mixture, it displays aggregate-induced emission (AIE) bands.
View Article and Find Full Text PDF5-PC as a Lipid Probe Molecule and as a Second Phospholipid in Binary Phospholipid Mixtures: Saturation Recovery EPR Studies.
Int J Mol Sci
November 2024
Department of Biophysics, Medical University of Lublin, 20-090 Lublin, Poland.
Mixtures of two phospholipids (PLs) with different main phase transition temperatures were investigated. Host PLs (HPLs) were represented by DMPC, DPPC, DSPC, and DMPE. The admixed PL was the spin-labeled phosphatidylcholine 5-PC(1-palmitoyl-2-(5-doxylstearoyl)phosphatidylcholine), with a unique opportunity to monitor the properties and the local environments of all admixed PL molecules using saturation recovery EPR methods.
View Article and Find Full Text PDFImpact of Pluronic F-127 on the Stability of Quercetin-Loaded Liposomes: Insights from DSC Preformulation Studies.
Materials (Basel)
November 2024
Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimioupolis Zografou, 15771 Athens, Greece.
The aim of the present study is to evaluate the stability of DMPC:Pluronic F-127 and DPPC:Pluronic F-127 liposomes, both with and without incorporated quercetin. Quercetin belongs to the class of flavonoids and has shown antioxidant, antiviral, anti-inflammatory, anti-cancer, and antimicrobial activities. Dynamic light scattering, electrophoretic light scattering, and differential scanning calorimetry (DSC) were utilized to investigate the cooperative behavior between liposomal components and its effect on stability.
View Article and Find Full Text PDFDesign optimization of Fucoidan-coating Cationic Liposomes for enhance Gemcitabine delivery.
Invest New Drugs
October 2024
School of Life Sciences and Technology, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China.
Obstacles facing chemotherapeutic drugs for cancers led scientists to load Gemcitabine (GEM) into nanocarriers like liposomes, known for their nontoxicity profile and targeting capacity. The liposomal nanostructures containing GEM were coated with Fucoidan (FU) due to its anti-tumor properties by targeting cancer cells. Thus four different cationic liposomes formulations were prepared by thin-film hydration method in optimal conditions: DOTAP (formulation A); DPPC/DOTAP (4:1 molar ratio, formulation B), DPPC/DMPC/DOTAP (4:1:1 molar ratio, formulation C) and DPPC/DMPC/DOTAP/DSPE-mPEG2000 (4:1:1:0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!