A slot blot enzyme-linked immunosorbent assay, using monoclonal antibodies specific only for Salmonella paratyphi A, to detect S. paratyphi A contamination in raw prawns has been established. When artificially contaminated prawn samples were tested. S. paratyphi A contamination could be identified correctly within 20 h. No false positives from samples artificially contaminated by other microorganisms were obtained. The sensitivity was such that as few as 1 S. paratyphi A organism per g of raw prawn could be detected. Therefore, the assay constituted a promising test for the rapid and specific detection of S. paratyphi A in prawns.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC202030 | PMC |
| http://dx.doi.org/10.1128/aem.60.12.4612-4613.1994 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biosynthesized ZnO NPs loaded-electrospun PVA/sodium alginate/glycine nanofibers: synthesis, spinning optimization and antimicrobial activity evaluation.
Sci Rep
January 2025
Chemistry Department, Faculty of Science, Menoufia University, Shibin El-Kom, 32511, Egypt.
In this work, microalgae-based zinc oxide nanoparticles loaded with electrospun polyvinyl alcohol (PVA)/sodium alginate (SA) nanofibers were fabricated by electro-spinner. PVA/SA fibrous mats were crosslinked by citric acid, which enhanced their thermal stability and swelling behavior. Green-synthesized ZnO NPs were laboratory synthesized and characterized by FTIR, XRD, EDX, SEM, TEM and TGA analyses.
View Article and Find Full Text PDFComplete genome sequence of X14-67 with antimicrobial properties isolated from aged "Chachiensis".
Microbiol Resour Announc
January 2025
School of Food Sciences and Engineering, South China University of Technology, Guangzhou, China.
X14-67 was isolated from "Chachiensis," the natural aged pericarps of Blanco. It had inhibitory activity against , , , and . Here, its genome was sequenced and annotated to understand its antimicrobial properties.
View Article and Find Full Text PDFGenomic diversity and potential transmission and persistence of Salmonella in the Cambodian vegetable supply chain.
J Food Prot
January 2025
Department of Food Science, The Pennsylvania State University, University Park, PA 16802. Electronic address:
S. enterica isolates (n = 78) obtained from the vegetable supply chain (farms, distribution centers, markets) in two Cambodian provinces (Siem Reap, Battambang) were sequenced and analyzed. In silico identification of serotypes and detection of antimicrobial resistance genes was performed using SISTR and ABRicate, respectively.
View Article and Find Full Text PDFA core glycolipid vaccine elicits cross-reactive antibodies against Salmonella spp. and protects against invasive nontyphoidal Salmonella disease in mice.
J Infect Dis
December 2024
Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Background: Enteric fever caused by Salmonella enterica serovars Typhi and Paratyphi A in addition to gastroenteritis and invasive disease, predominantly attributable to nontyphoidal Salmonella serovars Typhimurium and Enteritidis, are major causes of death and disability across the globe. A broad-spectrum vaccine that protects against disease caused by typhoidal and nontyphoidal serovars of Salmonella is not available for humans but would prevent a considerable burden of disease worldwide.
Methods: We previously developed a broad-spectrum vaccine for Gram-negative bacteria that is based on the inner core domain of detoxified Escherichia coli O111, Rc (J5) mutant lipooligosaccharide, a highly conserved antigen across Gram-negative bacteria, complexed with an outer membrane protein of group B Neisseria meningitidis.
MtlD as a therapeutic target for intestinal and systemic bacterial infections.
J Bacteriol
December 2024
Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.
Unlabelled: The ability to treat infections is threatened by the rapid emergence of antibiotic resistance among pathogenic microbes. Therefore, new antimicrobials are needed. Here we evaluate mannitol-1-phosphate 5-dehydrogenase (MtlD) as a potential new drug target.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!