The aim of this study was to compare 17 different Aujeszky's disease virus (ADV) isolates from clinical outbreaks of AD by using DNA biotinylated probes. All isolates were collected in Poland between 1984 and 1991. The restriction fragment pattern (RFP) analysis done by hybridization to NIA-3 DNA biotinylated probe indicated that all Polish ADV field strains can be classified as type I of Suid herpesvirus 1. Hybridization with BamHI fragment 7 and gI gene biotinylated probes revealed an unusual heterogeneity of BamHI fragment 7 in almost 50% of strains isolated in Poland. The nature of the molecular changes in this fragment will be discussed.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Proximity Proteomics Reveals USP44 Forms a Complex with BRCA2 in Neuroblastoma Cells and Is Required to Prevent Chromosome Breakage.
Biomedicines
December 2024
Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN 55905, USA.
Background/objectives: The enzyme ubiquitin-specific protease 44 (USP44) is a deubiquitinating enzyme with identified physiological roles as a tumor suppressor and an oncogene. While some binding partners and substrates are known for USP44, the identification of other interactions may improve our understanding of its role in cancer. We therefore performed a proximity biotinylation study that identified products of several known cancer genes that are associated with USP44, including a novel interaction between BRCA2 and USP44.
View Article and Find Full Text PDFHigh-sensitivity detection of DNA in tongue swab samples.
J Clin Microbiol
December 2024
Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
Article Synopsis
- Tongue swab (TS) sampling combined with quantitative PCR (qPCR) shows promise as an alternative to sputum testing for tuberculosis (TB) diagnosis, although previous studies indicated lower sensitivity.
- Two strategies were evaluated to enhance sensitivity: one involved centrifugation to concentrate MTB DNA, achieving 83% sensitivity and 100% specificity, while the other utilized sequence-specific magnetic capture, resulting in 90% sensitivity and 97% specificity.
- These findings suggest that with further development, these methods could lead to more accessible and effective TB testing options.
NORAD exacerbates metabolic dysfunction-associated steatotic liver disease development via the miR-511-3p/Rock2 axis and inhibits ubiquitin-mediated degradation of ROCK2.
Metabolism
December 2024
Department of Pathology, School of Basic Medical Sciences, Wannan Medical College, Wuhu, China; Postdoctoral Research Station of Clinical Medicine, Jinan University, Guangzhou, China. Electronic address:
Background & Aims: Abnormal regulation of lncRNA is strongly linked to metabolic dysfunction-associated steatotic liver disease (MASLD). However, the precise molecular mechanisms remain unclear. This study explores the roles of noncoding RNA activated by DNA damage (NORAD)/miR-511-3p/Rho-associated protein kinase 2 (Rock2) axis and the NORAD/ROCK2 interaction in the development of MASLD.
View Article and Find Full Text PDFNuclear Factor I family members are key transcription factors regulating gene expression.
Mol Cell Proteomics
November 2024
Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland; iCAN Digital Precision Cancer Medicine Flagship, University of Helsinki, Helsinki, Finland. Electronic address:
The Nuclear Factor I (NFI) family of transcription factors (TFs) plays key roles in cellular differentiation, proliferation, and homeostasis. As such, NFI family members engage in large number of interactions with other proteins and the chromatin. However, despite their well-established significance, the NFIs interactomes, their dynamics, and their functions have not been comprehensively examined.
View Article and Find Full Text PDFOpen architecture of archaea MCM and dsDNA complexes resolved using monodispersed streptavidin affinity CryoEM.
Nat Commun
November 2024
Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
The cryo-electron microscopy (cryoEM) method has enabled high-resolution structure determination of numerous biomolecules and complexes. Nevertheless, cryoEM sample preparation of challenging proteins and complexes, especially those with low abundance or with preferential orientation, remains a major hurdle. We developed an affinity-grid method employing monodispersed single particle streptavidin on a lipid monolayer to enhance particle absorption on the grid surface and alleviate sample exposure to the air-water interface.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!