Transcription of the mouse Upstream Binding Factor (mUBF) gene, that encodes one of the essential transcription factors for ribosomal DNA transcription, starts from several nucleotides. Neither typical TATA-box nor CCAAT-box is found upstream of the transcription initiation site. In the promoter region, there are eight GC-boxes, eight AP-2 binding consensus sequences, four cAMP response elements, and several serum response element equivalent sequences. These elements appear to play a positive role for the regulation of the mUBF gene as a whole. Among serum response elements, members located between -1182 and -343 are indeed responsive to serum, suggesting their important role in the high expression of mUBF under cell growth conditions.
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Regulation of mouse UBF gene by multiple growth-related control elements.
Biochem Biophys Res Commun
December 1994
Department of Biochemistry, University of Tokyo Faculty of Medicine, Japan.
Transcription of the mouse Upstream Binding Factor (mUBF) gene, that encodes one of the essential transcription factors for ribosomal DNA transcription, starts from several nucleotides. Neither typical TATA-box nor CCAAT-box is found upstream of the transcription initiation site. In the promoter region, there are eight GC-boxes, eight AP-2 binding consensus sequences, four cAMP response elements, and several serum response element equivalent sequences.
View Article and Find Full Text PDFLocalization of the RNA polymerase I transcription factor hUBF during the cell cycle.
J Cell Sci
February 1993
Institut Jacques Monod, Paris, France.
Autoantibodies directed against nucleoli that recognized a doublet of 97-94 kDa in HeLa nuclear protein extracts were identified. The two polypeptides bound equal amounts of antibody, and each was recognized by antibodies affinity purified using the other polypeptide. These antigens were localized in the secondary constriction of PtK1 cells, i.
View Article and Find Full Text PDFMouse rRNA gene transcription factor mUBF requires both HMG-box1 and an acidic tail for nucleolar accumulation: molecular analysis of the nucleolar targeting mechanism.
EMBO J
October 1992
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
RNA polymerase I requires at least two nucleolar transcription factors, UBF and SL-1, for ribosomal RNA gene (rDNA) transcription. UBF requires SL-1 for the formation of a stable initiation complex on the rDNA promoter region. We have determined the region of mouse UBF (mUBF) required for nucleolar targeting.
View Article and Find Full Text PDFThe nucleolar transcription factor mUBF is phosphorylated by casein kinase II in the C-terminal hyperacidic tail which is essential for transactivation.
EMBO J
June 1992
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.
UBF is a DNA binding protein which interacts with both the promoter and the enhancer of various vertebrate ribosomal RNA genes and functions as a transcription initiation factor for RNA polymerase I (pol I). We have purified murine UBF to apparent molecular homogeneity and demonstrate that its transactivating potential, but not its DNA binding activity, is modulated in response to cell growth. In vivo labelling experiments demonstrate that UBF is a phosphoprotein and that the phosphorylation state is different in growing and quiescent cells.
View Article and Find Full Text PDFCloning and structural analysis of cDNA and the gene for mouse transcription factor UBF.
Nucleic Acids Res
September 1991
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
The gene and protein structure of the mouse UBF (mUBF), a transcription factor for mouse ribosomal RNA gene, have been determined by cDNA and genomic clones. The unique mUBF gene consists of 21 exons spanning over 13 kb. Two mRNAs coding for mUBF1 and mUBF2 having 765 a.
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