As there are few data on primary structures of fish apolipoproteins (apo), we determined the N-terminal sequence of a 28 kDa major serum protein of rainbow trout, a possible homologue of apoAI protein in higher vertebrates. Rainbow trout serum was separated by two-dimensional electrophoresis. A spot of the 28 kDa protein (pl 5.45) was cut out and directly applied to a protein sequencer. We succeeded in sequencing 30 amino acids from the N-terminal. The sequence of the 28 kDa protein shared a high similarity with that of apoAI of Atlantic salmon (Salmo salar). These results showed that the 28 kDa protein is apoAI of rainbow trout.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses.
J Fish Dis
January 2025
Fish Disease Research Unit, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
Piscine orthoreovirus-1 and 3 (PRV-1, PRV-3) cause highly prevalent infection in cultured salmonids and can induce heart and skeletal muscle inflammation (HSMI) resulting in economic losses in aquaculture. However, to date, PRV-1 and PRV-3 have withstood replication in continuous cell lines. In this study, we used beating heart cell cultures obtained from different developmental stages of rainbow trout (Oncorhynchus mykiss) (RTC-L and RTC-A) and tested their ability to sustain replication of PRV-1 and PRV-3.
View Article and Find Full Text PDFDouble Trouble for Native Species Under Climate Change: Habitat Loss and Increased Environmental Overlap With Non-Native Species.
Glob Chang Biol
January 2025
Department of Fisheries, Wildlife, and Conservation Sciences, Oregon State University, Corvallis, Oregon, USA.
Climate change and biological invasions are affecting natural ecosystems globally. The effects of these stressors on native species' biogeography have been studied separately, but their combined effects remain overlooked. Here, we develop a framework to assess how climate change influences both the range and niche overlap of native and non-native species using ecological niche models.
View Article and Find Full Text PDFComparison of in vitro transcriptomic points of departure with fish acute and chronic toxicity values: A case study of rainbow trout cells exposed to pesticides.
Environ Toxicol Chem
January 2025
Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Quebec, Canada.
There is growing interest in transcriptomic points of departure (tPOD) values from in vitro experiments as an alternative to animal test method. The study objective was to calculate tPODs in rainbow trout gill cells (RTgill-W1 following OECD 249) exposed to pesticides, and to evaluate how these values compare to fish acute and chronic toxicity data. Cells were exposed to one fungicide (chlorothalonil), ten herbicides (atrazine, glyphosate, imazethapyr, metolachlor, diquat, s-metolachlor, AMPA, dicamba, dimethenamid-P, metribuzin), eight insecticides (chlorpyrifos, diazinon, permethrin, carbaryl, clothianidin, imidacloprid, thiamethoxam, chlorantraniliprole), and OECD 249 positive control 3,4-dichloroaniline.
View Article and Find Full Text PDFAccounting for the role of the gastro-intestinal tract in the ammonia and urea-N dynamics of freshwater rainbow trout on long-term satiation feeding.
J Exp Biol
January 2025
Department of Zoology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
The contribution of the gut to the ingestion, production, absorption, and excretion of the extra ammonia and urea-N associated with feeding ("exogenous" fraction) has received limited prior attention. Analysis of commercial pellet food revealed appreciable concentrations of ammonia and urea-N. Long term satiation-feeding increased whole trout ammonia and urea-N excretion rates by 2.
View Article and Find Full Text PDFTransient cognitive impacts of oxygen deprivation caused by catch-and-release angling.
Biol Lett
January 2025
Centre for Ecology and Evolution in Microbial Model Systems (EEMiS), Department of Biology and Environmental Science, Faculty of Health and Life Sciences, Linnaeus University, Kalmar 39231, Sweden.
Vertebrate brain function is particularly sensitive to the effects of hypoxia, with even brief periods of oxygen deprivation causing significant brain damage and impaired cognitive abilities. This study is the first to investigate the cognitive consequences of hypoxia in fish, specifically induced by exhaustive exercise and air exposure, conditions commonly encountered during catch-and-release (C&R) practices in recreational fishing. Angling exerts substantial pressure on inland fish populations, underscoring the need for sustainable practices like C&R.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!