The presence of plasma membrane Cl- channels was investigated in brown-fat cells differentiated in culture. Single channel activity was followed by the patch-clamp technique, with an NMDG+ Cl- pipette solution and a NaCl bath solution. Only rarely was putative Cl- channel activity encountered in the cell-attached mode. However, after excision, ion channel activity of two types was observed in inside-out patches: one type represented the earlier observed non-selective cation channel, and one a putative Cl- channel, with the following characteristics: practically non-rectifying current-voltage relationship with a conductance of approximately 50 pS and a reversal potential of approximately 0 in symmetrical Cl- solutions, voltage dependent channel activity (Po approached 0.5 at positive holding potentials), and rapid flickering activity in the open state. It is discussed whether this Cl- channel may be responsible for the initial depolarization phase observed after alpha 1-adrenergic stimulation of brown-fat cells.
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