The RAD16 gene product has been shown to be essential for the repair of the silenced mating type loci [Bang et al. (1992) Nucleic Acids Res. 20, 3925-3931]. More recently we demonstrated that the RAD16 and RAD7 proteins are also required for repair of non-transcribed strands of active genes in Saccharomyces cerevisiae [Waters et al. (1993) Mol. Gen. Genet. 239, 28-32]. We have studied the regulation of the RAD16 gene and found that the RAD16 transcript levels increased up to 7-fold upon UV irradiation. Heat shock at 42 degrees C also results in elevated levels of RAD16 mRNA. In sporulating MAT alpha/MATa diploid cells RAD16 mRNA is also induced. The basal level of the RAD16 transcript is constant during the mitotic cell cycle. G1-arrested cells show normal induction of RAD16 mRNA upon UV irradiation demonstrating that the induction is not a secondary consequence of G2 cell cycle arrest following UV irradiation. However, in cells arrested in G1 the induction of RAD16 mRNA after UV irradiation is not followed by a rapid decline as occurs in normal growing cells suggesting that the down regulation of RAD16 transcription is dependent on progression into the cell cycle.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC306921 | PMC |
| http://dx.doi.org/10.1093/nar/23.10.1679 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Differentiation of bone marrow stromal cells into osteoblasts in a self-assembling peptide hydrogel: in vitro and in vivo studies.
J Biomater Appl
March 2011
Oral Implantology and Regenerative Dental Medicine Graduate School, Tokyo Medical and Dental University 1-5-45 Yushima, Bunkyo-ku, Tokyo, Japan.
Article Synopsis
- - This study investigates RAD16, a synthetic peptide biomaterial that self-assembles into a hydrogel, for use as a scaffold in tissue engineering to support bone cell growth and differentiation.
- - RAD16 was cocultured with bone marrow cells from rats, revealing that the addition of dexamethasone enhanced cell proliferation and osteogenic differentiation, as indicated by increases in alkaline phosphatase activity and specific gene expressions.
- - While the RAD16 combined with hydroxyapatite (HA) particles showed improved cell growth and gene expression related to osteogenesis, no ectopic mineralization occurred during the experiment, emphasizing the need for further exploration and modifications of RAD16 for effective tissue engineering.
Cloning and sequence of the LYS2 homologue gene from the osmotolerant yeast Pichia sorbitophila.
Yeast
January 2001
Laboratoire de Microbiologie et Génétique, UPRES-A 7010, Université Louis Pasteur/CNRS, 28 rue Goethe, F-67083 Strasbourg, France.
We have isolated the Pichia sorbitophila LYS2 (PsLYS2) gene by complementation of a lys2 Saccharomyces cerevisiae mutant. The sequenced DNA fragment contains a putative ORF of 4197 bp and the deduced translation product shares a global identity of 66% and 58% to the Lys2 protein homologues of Candida albicans and S. cerevisiae, respectively.
View Article and Find Full Text PDFRecovery of RNA polymerase II synthesis following DNA damage in mutants of Saccharomyces cerevisiae defective in nucleotide excision repair.
Nucleic Acids Res
November 1997
Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA.
We have measured the kinetics of the recovery of mRNA synthesis in the inducible GAL10 and RNR3 genes after exposure of yeast cells to ultraviolet (UV) radiation. Such recovery is abolished in mutant strains defective in nucleotide excision repair (NER) of DNA, including a rad23 mutant. Mutants defective in the RAD7 or RAD16 genes, which are required for the repair of the non-transcribed strand but not the transcribed strand of transcriptionally active genes, show slightly faster recovery of RNA synthesis than wild-type strains.
View Article and Find Full Text PDFExcision repair at the level of the nucleotide in the Saccharomyces cerevisiae MFA2 gene: mapping of where enhanced repair in the transcribed strand begins or ends and identification of only a partial rad16 requisite for repairing upstream control sequences.
J Mol Biol
March 1997
School of Biological Sciences University of Wales Swansea Singleton Park, UK.
We wished to determine where transcription enhanced nucleotide excision repair begins and ends for a Saccharomyces cerevisiae gene transcribed by RNA polymerase II, and to examine the role of the RAD16 gene in repairing upstream, non-transcribed control sequences of such a gene. To do so, we developed a method to study the repair of UV induced cyclobutane pyrimidine dimers (CPDs) at the level of the nucleotide in the control and coding sequences of the MFA2 gene. This gene is active in haploid a mating type cells but inactive in alpha cells: its regulation is mediated by changes in chromatin structure.
View Article and Find Full Text PDFDevelopmental regulation of Zbu1, a DNA-binding member of the SWI2/SNF2 family.
Dev Biol
March 1997
Cardiovascular Research Center, Massachusetts General Hospital-East, Charlestown 02129, USA.
The SWI2/SNF2 gene family has been implicated in a wide variety of processes, involving regulation of DNA structure and chromatin configuration, mitotic chromosome segregation, and DNA repair. Here we report the characterization of the Zbu1 gene, also known as HIP116, located on human chromosome band 3q25, which encodes a DNA-binding member of this superfamily. Zbu1 was isolated in this study by its affinity for a site in the myosin light chain 1/3 enhancer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!