Objectives: This in vitro study aimed to examine the depth of preparation and incidence of dentine exposure resulting from the use of a 'freehand' technique to prepare maxillary central incisors for porcelain veneers.
Methods: Twenty-two maxillary central incisors were selected. Prior to preparation an index of the labial surface of each tooth was recorded and the tooth secured in a jig to permit accurate relocation. Two operators each prepared 11 teeth aiming to reduce the labial thickness evenly by 0.5 mm. Low viscosity silicone impression material was then placed on the index and the teeth relocated into the jig. Upon removal, this material was sectioned in the cervical, middle and incisal thirds of the tooth and its thickness measured using a toolmaker's microscope. The teeth were acid etched and also stained with a dentine dye to identify any area of dentine exposed during preparation.
Results: Significant differences (P < 0.001) in the depth of preparation at different sites, with least reduction in the mid-incisal region, were found. Greater reduction was found at the cervical and proximal margins with areas of dentine exposed at those sites in the majority of teeth.
Conclusions: In view of the incidence and position of dentine exposure found in this study, the use of a dentine bonding system during the placement of porcelain veneers would appear essential when employing a 'freehand' preparation technique.
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Nat Methods
January 2025
Research Institute of Molecular Pathology (IMP), Vienna BioCenter, Vienna, Austria.
Despite significant advancements in sample preparation, instrumentation and data analysis, single-cell proteomics is currently limited by proteomic depth and quantitative performance. Here we demonstrate highly improved depth of proteome coverage as well as accuracy and precision for quantification of ultra-low input amounts. Using a tailored library, we identify up to 7,400 protein groups from as little as 250 pg of HeLa cell peptides at a throughput of 50 samples per day.
View Article and Find Full Text PDFNat Protoc
January 2025
Advanced Research Support Center, Ehime University, Ehime, Japan.
Top-down analysis of intact proteins and middle-down analysis of proteins subjected to limited digestion require efficient detection of traces of proteoforms in samples, necessitating the reduction of sample complexity by thorough pre-fractionation of the proteome components in the sample. SDS-PAGE is a simple and inexpensive high-resolution protein-separation technique widely used in biochemical and molecular biology experiments. Although its effectiveness for sample preparation in bottom-up proteomics has been proven, establishing a method for highly efficient recovery of intact proteins from the gel matrix has long been a challenge for its implementation in top-down and middle-down proteomics.
View Article and Find Full Text PDFNutr Health
January 2025
Department of Nutrition and Health, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Background: Nutrition assessment is the first step of the nutrition care process (NCP), which includes investigating physical status and malnutrition signs. With the widespread digitalization of healthcare in Indonesia, including telehealth in dietetic practice (telenutrition), evidence is required to address the barriers and improve the current practice of conducting remote physical assessments.
Aim: This study aimed to explore the adaptation of nutrition-focused physical examination (NFPE) using telenutrition in Indonesia.
Anal Chem
January 2025
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.
Lipid A, a well-known saccharolipid, acts as the inner lipid-glycan anchor of lipopolysaccharides in Gram-negative bacterial cell membranes and functions as an endotoxin. Its structure is composed of two glucosamines with β(1 → 6) linkages and various fatty acyl and phosphate groups. The lipid A structure can be used for the identification of bacterial species, but its complexity poses significant structural characterization challenges.
View Article and Find Full Text PDFConventional laser micromachining technologies rely on trial-and-error optimization to obtain precise surface geometry. In this study, we present a laser micromachining setup that enables the preparation of the desired surface geometry without the need for parameter exploration. The setup consists of a laser scanning system, a coaxial imaging system, a paraxial laser line projector, and a three-axis stage.
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