The primary structure of human sorbitol dehydrogenase (SORD) was determined by cDNA and genomic cloning. The nucleotide sequence of the mRNA covers 2471 bp including an open reading frame that yields a protein of 356 amino acid residues. The gene structure of SORD spans approximately 30 kb divided into 9 exons and 8 introns. The gene was localized to chromosome 15q21.1 by in situ hybridization. Two transcription initiation sites were detected. Three Sp1 sites and a repetitive sequence (CAAA)5 were observed in the 5' noncoding region; no classical TATAA or CCAAT elements were found. The related alcohol dehydrogenases and zeta-crystallin have the same gene organization split by 8 introns, but no splice points coincide between SORD and these gene types. The deduced amino acid sequence of the SORD structure differs at a few positions from the directly determined protein sequence, suggesting allelic forms of the enzyme. High levels of SORD transcripts were observed in lens and kidney, as judged from Northern blot analysis.
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Drug Discov Ther
January 2025
Department of Cardiovascular Diseases, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
Several sodium-glucose cotransporter 2 (SGLT2) inhibitors are known to have beneficial effects on renal function in patients with type 2 diabetes. However, the long-term effects of luseogliflozin, an SGLT2 inhibitor, remain uncertain in real-world settings. This multicenter, open-label, prospective observational study evaluated the long-term effects of luseogliflozin on renal function in Japanese patients with type 2 diabetes.
View Article and Find Full Text PDFTrans R Soc Trop Med Hyg
January 2025
Department of Microbiology, Kogi State University, P.M.B. 1008 Anyigba, Nigeria.
Background: Globally, diarrhoeagenic Escherichia coli (DEC) has been implicated in the spread of waterborne diseases and abattoir wastewater has played a role in its dissemination into watersheds. This study isolated and characterised DEC from the abattoir wastewater-impacted Iyi-Etu River and other water sources at the Amansea livestock market settlement.
Methods: A total of 96 water samples comprising river water (upstream, downstream 1, downstream 2), borehole, well, sachet and abattoir wastewater samples were tested for DEC.
Parasit Vectors
December 2024
Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Background: Meglumine antimoniate is used to treat canine leishmaniosis. In humans, it has been associated with pancreatitis. Although a few case reports have described acute pancreatitis secondary to antimonial treatment in dogs, some studies have concluded that pancreatitis is not an adverse effect of this medication.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Department of Dairy and Food Science, South Dakota State University, Brookings, SD 57007, USA. Electronic address:
Plastic packaging has increased concerns about human health and the ecosystem due to non-biodegradability. Several biopolymers, such as cellulose, starch, and proteins, are being explored, and cellulosic residue from agricultural biomass is suitable to overcome this predicament. Herein, cellulosic residue fibers (ACR) extracted from alfalfa were used to prepare biodegradable films by solubilizing them in ZnCl solution and crosslinking the chains with calcium ions (Ca) and sorbitol.
View Article and Find Full Text PDFStem Cells Dev
December 2024
Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
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