Structural organization of the human sorbitol dehydrogenase gene (SORD).

Genomics

Laboratory of Mechanisms of Ocular Diseases, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Published: March 1995

The primary structure of human sorbitol dehydrogenase (SORD) was determined by cDNA and genomic cloning. The nucleotide sequence of the mRNA covers 2471 bp including an open reading frame that yields a protein of 356 amino acid residues. The gene structure of SORD spans approximately 30 kb divided into 9 exons and 8 introns. The gene was localized to chromosome 15q21.1 by in situ hybridization. Two transcription initiation sites were detected. Three Sp1 sites and a repetitive sequence (CAAA)5 were observed in the 5' noncoding region; no classical TATAA or CCAAT elements were found. The related alcohol dehydrogenases and zeta-crystallin have the same gene organization split by 8 introns, but no splice points coincide between SORD and these gene types. The deduced amino acid sequence of the SORD structure differs at a few positions from the directly determined protein sequence, suggesting allelic forms of the enzyme. High levels of SORD transcripts were observed in lens and kidney, as judged from Northern blot analysis.

Download full-text PDF

Source
http://dx.doi.org/10.1016/0888-7543(95)80082-wDOI Listing

Publication Analysis

Top Keywords

human sorbitol
8
sorbitol dehydrogenase
8
amino acid
8
sord
6
gene
5
structural organization
4
organization human
4
dehydrogenase gene
4
gene sord
4
sord primary
4

Similar Publications

Several sodium-glucose cotransporter 2 (SGLT2) inhibitors are known to have beneficial effects on renal function in patients with type 2 diabetes. However, the long-term effects of luseogliflozin, an SGLT2 inhibitor, remain uncertain in real-world settings. This multicenter, open-label, prospective observational study evaluated the long-term effects of luseogliflozin on renal function in Japanese patients with type 2 diabetes.

View Article and Find Full Text PDF

Background: Globally, diarrhoeagenic Escherichia coli (DEC) has been implicated in the spread of waterborne diseases and abattoir wastewater has played a role in its dissemination into watersheds. This study isolated and characterised DEC from the abattoir wastewater-impacted Iyi-Etu River and other water sources at the Amansea livestock market settlement.

Methods: A total of 96 water samples comprising river water (upstream, downstream 1, downstream 2), borehole, well, sachet and abattoir wastewater samples were tested for DEC.

View Article and Find Full Text PDF

Background: Meglumine antimoniate is used to treat canine leishmaniosis. In humans, it has been associated with pancreatitis. Although a few case reports have described acute pancreatitis secondary to antimonial treatment in dogs, some studies have concluded that pancreatitis is not an adverse effect of this medication.

View Article and Find Full Text PDF

Use of alfalfa cellulose for formulation of strong, biodegradable film to extend the shelf life of strawberries.

Int J Biol Macromol

December 2024

Department of Dairy and Food Science, South Dakota State University, Brookings, SD 57007, USA. Electronic address:

Plastic packaging has increased concerns about human health and the ecosystem due to non-biodegradability. Several biopolymers, such as cellulose, starch, and proteins, are being explored, and cellulosic residue from agricultural biomass is suitable to overcome this predicament. Herein, cellulosic residue fibers (ACR) extracted from alfalfa were used to prepare biodegradable films by solubilizing them in ZnCl solution and crosslinking the chains with calcium ions (Ca) and sorbitol.

View Article and Find Full Text PDF
Article Synopsis
  • α-synuclein (α-syn) is primarily found in red blood cells (erythrocytes), but its specific role in the production of these cells (erythropoiesis) is not well understood.
  • Researchers cultured erythroid cells from human umbilical cord progenitors and found that inhibiting α-syn increased erythropoiesis, as shown by changes in important surface markers and enzyme methylation.
  • The study also identified shifts in cellular metabolism, indicating that α-syn functions as a regulator of methylation that influences enzymes in fructose and mannose metabolism, thereby playing a crucial role in the formation of red blood cells.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!