In this paper we describe a novel method of cloning representative members of different repetitive DNA families based on the screening of a rice (Oryza sativa) genomic library using DNA from different C0t fractions as probes. Two genomic clones, which represent two different repetitive DNA families in rice, were isolated by this method and have been characterized. Their nucleotide sequences, copy numbers, distributions in major rice genome types, methylation patterns, and chromosomal localizations were determined.
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