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Proteomic interrogation of complex biomedical samples using the rapid denaturing organic digestion (DOD) method.
J Proteomics
February 2025
Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, United Kingdom; Vibrat-Ion Ltd, Aberystwyth, Wales, SY23 3AF, United Kingdom.
Article Synopsis
- Current aqueous-based tryptic digestion methods face challenges like long digestion times and variability in peptide identification and sequence coverage.
- The Denaturing Organic Digestion (DOD) method offers a faster alternative, requiring only common solvents and reagents, without needing expensive equipment.
- Results showed that the DOD method generated similar peptide profiles to traditional methods but identified more hydrophilic peptides; longer digestion times improved precision and peptide identification.
APEX1 in intestinal epithelium triggers neutrophil infiltration and intestinal barrier damage in ulcerative colitis.
Free Radic Biol Med
November 2024
Cancer Center of Daping Hospital Army Medical University, Chongqing 400042, China. Electronic address:
Article Synopsis
- Ulcerative colitis (UC) generates reactive oxygen species and DNA damage, with base excision repair (BER) enzymes like APEX1 playing a crucial role in maintaining DNA integrity, yet their specific relationship with UC is not well understood.
- *A study on colon tissue from UC patients revealed that APEX1 expression is linked to the severity of inflammatory bowel disease and neutrophil activity, suggesting it influences inflammatory processes in UC.
- *APEX1 deficiency leads to reduced chemokine production and neutrophil activation, alleviating colitis symptoms, which highlights APEX1's potential as a target for clinical therapies in treating IBD.*
Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis.
Int Immunopharmacol
September 2024
Cancer Center, Daping Hospital, Army Medical University, Chongqing 400042, China; Department of Oncology, The Fifth People's Hospital of Chongqing, Chongqing 400062, China. Electronic address:
The resistance of osteosarcoma (OS) to ionizing radiation (IR) is an obstacle for effective patient treatment. Apurinic/apyrimidinic endonuclease-reduction/oxidation factor 1 (APE1/Ref-1) is a multifunctional protein with DNA repair and reduction/oxidation (redox) activities. We previously revealed the role of APE1 in OS radioresistance; however, whether the redox activity of APE1 is involved in OS radioresistance is unclear.
View Article and Find Full Text PDFActivation of APE1 modulates Nrf2 protected against acute liver injury by inhibit hepatocyte ferroptosis and promote hepatocyte autophagy.
Int Immunopharmacol
February 2024
Center of TCM Preventive Treatment, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510315, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China. Electronic address:
Background: Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref-1) plays a crucial role in DNA base excision repair, cell apoptosis, cell signaling, and the regulation of transcription factors through redox modulation and the control of reactive oxygen species (ROS). However, the connection between APE1 and acute liver injury (ALI) remains enigmatic. This study aims to unravel the molecular mechanisms underlying ALI and shed light on the role of APE1 in this context.
View Article and Find Full Text PDFGreater socioenvironmental risk factors and higher chronic pain stage are associated with thinner bilateral temporal lobes.
Brain Behav
December 2023
Pain Research & Intervention Center of Excellence, University of Florida, Gainesville, Florida, USA.
Introduction: Previous research indicates ethnic/race group differences in pain and neurodegenerative diseases. Accounting for socioenvironmental factors reduces ethnic/race group differences in clinical and experimental pain. In the current study sample, we previously reported that in individuals with knee pain, ethnic/race group differences were observed in bilateral temporal lobe thickness, areas of the brain associated with risk for Alzheimer's disease, and related dementias.
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