The purpose of this study was to develop a model of gastrointestinal carcinogenesis using C57BL/6 mice. Treatment regimens consisted of one control group and 2 groups which received N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in drinking water: 50 micrograms/ml x 52 weeks and 100 micrograms/ml x 27 weeks. In addition, 2 protocols using adjuvant agents intended to increase tumor formation were used: MNNG (100 micrograms/ml) x 27 weeks + 0.2% taurocholic acid added to the diet from weeks 13-52, and MNNG (50 micrograms/ml) x 33 weeks+caerulein (10 micrograms/kg) subcutaneously 3 times/week from weeks 21-52. High-grade dysplasia was observed in the duodenum of 1/13 mice treated with MNNG (50 micrograms/ml). The combination of the latter and caerulein did not augment tumorigenesis. Mice treated with MNNG (100 micrograms/ml) frequently developed neoplasia in the duodenum and upper jejunum. Foci of low-grade and high-grade dysplasia alone were found in 3/12 (25%) mice; and intramucosal and invasive adenocarcinoma were found in 7/12 (58.3%) mice. The addition of taurocholic acid significantly increased the number and histological stages of the tumors (adenocarcinoma occurred in 100%, P = 0.03) and decreased the time for tumor formation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0304-3835(95)03748-l | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Deep vein thrombosis following below knee immobilization: the need for chemoprophylaxis.
Trauma Mon
December 2013
Department of Emergency Medicine, Shahid Beheshti University of Medical Sciences, Imam Hossein Hospital, Tehran, IR Iran.
Background: There is controversy regarding routine prophylaxis for deep vein thrombosis (DVT) in patients treated via a short leg cast or splint following lower extremity trauma.
Objectives: The main aim of this study is to evaluate the incidence of DVT and need for chemoprophylaxis in these patients.
Materials And Methods: Patients with ankle sprains or stable foot/ankle fractures were entered in this cross-sectional study.
Immunogenicity and safety of a pentavalent diphtheria, tetanus, acellular pertussis, inactivated poliovirus, Haemophilus influenzae type B conjugate combination vaccine (Pentaxim) with hepatitis B vaccine.
Indian Pediatr
November 2009
Lady Hardinge Medical College and Associated Hospitals, New Delhi, India.
Objective: To obtain immunogenicity and safety data for a pentavalent combination vaccine (diphtheria, tetanus, acellular pertussis, inactivated poliovirus, Hib polysaccharide-conjugate).
Design: Multicenter, open, Phase III clinical study. A DTaP-IPV//PRP approximately T vaccine (Pentaxim) was given at 6,10,14 weeks of age; and Hepatitis B vaccine at 0,6,14 or at 6,10,14 weeks of age.
Activation of the fibrinolytic cascade early in pregnancy among women with spontaneous preterm birth.
Obstet Gynecol
November 2008
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Magee Womens Research Institute, Pittsburgh, Pennsylvania 15213, USA.
Objective: To evaluate the association of early pregnancy concentrations of thrombin-antithrombin III complex with subsequent spontaneous preterm birth.
Methods: In a nested case-control study, thrombin-antithrombin III complex was measured in plasma before 20 weeks of gestation (mean 9.9 weeks) among women without chronic conditions, preeclampsia, or growth restriction.
Duration of intrapartum prophylaxis and concentration of penicillin G in fetal serum at delivery.
Obstet Gynecol
August 2008
Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut, USA.
Objective: Intrapartum penicillin G prophylaxis aims to prevent early-onset group B streptococci (GBS) sepsis by interrupting vertical transmission. We examined the relationship between duration of prophylaxis and fetal serum penicillin G levels among fetuses exposed to fewer than 4 hours of prophylaxis compared with longer durations.
Methods: In this prospective cohort study, 98 laboring GBS-positive women carrying singleton gestations at 37 weeks or greater were administered 5 million units of intravenous penicillin G followed by 2.
Variable mannose-binding lectin expression during postoperative acute-phase response.
Surg Infect (Larchmt)
October 2006
Department of Surgery, Academic Medical Center Amsterdam, University of Amsterdam, The Netherlands.
Background: Low plasma concentrations and genetic polymorphisms of mannan-binding lectin (MBL) have been associated with infectious disease complications during various conditions. The present study examined the nature and expression of MBL deficiency during a surgery-induced acute-phase response.
Methods: Blood was sampled from 20 consecutive patients before and 1, 3, 5, 7, and 10 days and 6 weeks after a uniform abdominal operation (transhiatal esophagectomy).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!