Competitive methods of enzyme immuno assay (EIA) for detecting simazine and atrazine were developed, and conditions providing optimal performance were found. EIA sensitivity was shown to increase by an order of magnitude if samples were preincubated with antibodies; chloride ions were omitted; a herbicide-peroxidase conjugate was treated with urea. In EIA using labelled antibodies sensitivities thresholds towards simazine and atrazine were 0.05 and 0.1 ng/ml, respectively. EIA took 1-2 h to be done. The methods developed might be applied for quality control of water.
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