A new artificial carrier particle agglutination test using polyamino acid (polyamino PA) was developed for anti HTLV-I assay. We carried out the comparative study on anti-HTLV-I among polyamino PA, gelatin PA, EIA and WB methods. All of 76 ATL, 20 HAM/TSP, 53 patients with uveitis and 50 HTLV-I carriers were seropositive and 50 HTLV-I non carriers were seronegative with four methods. Eighteen of 503 patients include autoimmune diseases showed seropositive by polyamino PA and gelatin PA. One of 19 seropositives by EIA was false positive. All of 25 sera showed non-specific reaction by the gelatin PA were clearly negative by the polyamino PA. This is due to the fact that the polyamino acid particle has a greater specific gravity as carrier. The final judgement was got within 45 minutes. It was earlier more 30 minutes than the gelatin PA. The polyamino PA is a simple, rapid, sensitive and specific method. Therefore, it is useful for mass screening and clinical diagnosis.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Thermal aggregation of immunoglobulin G depending on the charge state of protein-polyelectrolyte complexes.
Int J Biol Macromol
January 2025
Institute of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan. Electronic address:
Protein-polyelectrolyte complexes (PPCs) exhibit opposite effects, both suppressing and promoting protein aggregation, depending on the type of components. For the application of PPCs as protein stabilization technology, these opposite effects must be controlled. In this study, we investigated the thermal aggregation of immunoglobulin G (IgG)-polyamino acid complexes to elucidate the relationship between the charge state of PPCs, evaluated by the zeta potential, and their opposite effects on the aggregation process, which were measured using a spectrophotometer.
View Article and Find Full Text PDFThioether functionalized degradable poly(amino acids) and its calcium sulfate/calcium hydrogen phosphate composites: Reducing oxidative stress and promoting osteogenesis.
Colloids Surf B Biointerfaces
April 2025
College of Physical, Sichuan University, Chengdu, Sichuan 610065, PR China. Electronic address:
The imbalance of redox homeostasis, especially the abnormal levels of reactive oxygen species (ROS), is a key obstacle in the bone repair process. Therefore, developing materials capable of scavenging ROS and modulating the microenvironment of bone defects is crucial for promoting bone repair. In this study, to endow poly(amino acids) (PAA) and its composites with anti-oxidative stress properties and enhanced osteogenic differentiation, we designed and prepared a calcium sulfate/calcium hydrogen phosphate/poly(amino acids) (PCDM) composite material with a thioether structure (-S-) in the molecular chain of PAA matrix through situ polymerization and physical blending method.
View Article and Find Full Text PDFRecent advances in poly(amino acids), polypeptides, and their derivatives in drug delivery.
Nanoscale
January 2025
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen 361005, China.
Poly(amino acids), polypeptides, and their derivatives have demonstrated significant potential as biodegradable biomaterials in the field of drug delivery. As degradable drug carriers, they can effectively load or conjugate drug molecules including small molecule drugs, nucleic acids, peptides, and protein-based drugs, enhancing the stability and targeting of the drugs . This strategy ultimately facilitates precise drug delivery and controlled release, thereby improving therapeutic efficacy and reducing side effects within the body.
View Article and Find Full Text PDFMicroscale droplet assembly enables biocompatible multifunctional modular iontronics.
Science
November 2024
Department of Chemistry, University of Oxford, Oxford, UK.
Hydrogel iontronic devices can emulate biological functions and communicate with living matter. But the fabrication of miniature, soft iontronic devices according to modular designs has not been achieved. In this work, we report the use of surfactant-supported assembly of freestanding microscale hydrogel droplets to construct various iontronic modules, circuits, and biointerfaces.
View Article and Find Full Text PDFPolyamino Acid Based Zwitterionic Coating can Inhibit Coagulation and Inflammation Through Anti-Fouling and Restoring Microenvironment.
Macromol Biosci
January 2025
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518000, China.
Protein adhesion and thrombosis formation caused by limited surface properties pose great challenges to biomedical implants. Although various hydrophilic coating or drug release coatings are reported, the single coating cannot cope with cases under the condition of complex physiological environment, which causes the coating effect is limited. In this study, a polyamino acid-derived zwitterionic coating is constructed to eliminate reactive oxygen species (ROS) in the microenvironment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!