Antigenicity of outer membrane proteins was studied and compared between Kanagawa positive (clinical) and negative (environmental) strains of Vibrio parahaemolyticus. Murine antibodies recognized a wide range of outer membrane proteins of Kanagawa negative strains as antigens with molecular masses ranging between 102 kDa to 14 kDa. However, only a few of the total outer membrane proteins of clinical isolates were antigenic and comprised a 55kDa protein as the major antigen. Although a marked difference in antigenicity was found, molecular masses of outer membrane proteins of Kanagawa positive and negative strains migrated similarly in SDS-PAGE. Several outer membrane proteins of Kanagawa-positive strains were found to be antigenic and ubiquitous in a cross-reactivity study indicating that the ubiquitous proteins which could not be recognized by anti-KP antibodies were buried in the outer membrane.
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http://dx.doi.org/10.1016/s0934-8840(11)80334-8 | DOI Listing |
Vertebrate vision in dim-light environments is initiated by rod photoreceptor cells that express the photopigment rhodopsin, a G-protein coupled receptor (GPCR). To ensure efficient light capture, rhodopsin is densely packed into hundreds of membrane discs that are tightly stacked within the rod-shaped outer segment compartment. Along with its role in eliciting the visual response, rhodopsin serves as both a building block necessary for proper outer segment formation as well as a trafficking guide for a few outer segment resident membrane proteins.
View Article and Find Full Text PDFACS Synth Biol
January 2025
Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan.
Bacterial outer membrane vesicles (OMVs) have emerged as promising vehicles for anticancer drug delivery due to their inherent tumor tropism, immune-stimulatory properties, and potential for functionalization with therapeutic proteins. Despite their advantages, the high lipopolysaccharide (LPS) endotoxin content in the OMVs raises significant safety and regulatory challenges. In this work, we produce LPS-attenuated and LPS-free OMVs and systematically assess the effects of LPS modification on OMVs' physicochemical characteristics, membrane protein content, immune-stimulatory capacity, tolerability, and anticancer efficacy.
View Article and Find Full Text PDFActa Trop
January 2025
Professor, Department of Paediatrics, All India Institute of Medical Sciences [AIIMS], Bhubaneswar, Odisha.751019. Electronic address:
Spotted fever group Rickettsia (SFGR) infections remain largely under-investigated as causative agents of acute undifferentiated febrile illness (AUFI) in resource-limited settings. Few studies are available on the prevalence of SFGR infections in India, especially in eastern India. In a cross-sectional study conducted in 192 hospitalized adult and paediatric patients with AUFI, the frequency of SFGR using sequential PCR targeting genes encoding citrate synthase gene (gltA), 17 kDa lipoprotein precursor antigen (17kDa), outer membrane proteins A and B (omp A & omp B) was 6.
View Article and Find Full Text PDFJ Trop Med
December 2024
Department of Microbiology, Agricultural Research, Education and Extension Organization (AREEO), Razi Vaccine and Serum Research Institute, Karaj, Iran.
The protein is highly conserved among pathogenic serovars and it is expressed during both acute and chronic infections. The aim of this study was to clone and sequence of the protein-encoding gene of serovars. In this study, 23 pathogenic serovars and two nonpathogenic serovars were used.
View Article and Find Full Text PDFJ Pharm Anal
December 2024
Institute of Infectious Disease and Infection Control, Jena University Hospital, Jena, 07747, Germany.
In our prior research, polymer nanoparticles (NPs) containing tobramycin displayed robust antibacterial efficacy against biofilm-embedded () and (. ) cells, critical pathogens in cystic fibrosis. In the current study, we investigated the deposition of a nanoparticulate carrier composed of poly(d,l-lactic--glycolic acid) (PLGA) and poly(ethylene glycol)--PLGA (PEG-PLGA) that was either covalently bonded with cyanine-5-amine (Cy5) or noncovalently bound with freely embedded cationic rhodamine B (RhB), which served as a drug surrogate.
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