A sampling technique for the determination of chloroform in alveolar breath is presented. Subjects exhale in a homemade device for collection of the alveolar fraction. The collected sample is transferred to a partially evacuated headspace vial and subsequently analyzed by capillary gas chromatography using electron-capture detection. Concentrations greater than 50 micrograms/m3 can readily be detected. Good correlation observed between chloroform levels in alveolar breath and plasma validates the sampling technique.
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