Three modifications of quantitative enzyme immunoassay (EIA) for assessment of Aujeszky's disease virus (ADV) in culture medium are compared, in which monoclonal antibodies (MAB) to ADV GH glycoprotein were used: nonconcurrent two-site "sandwich" EIA on the basis of two different MABs, indirect concurrent EIA, and direct concurrent EIA. MABs fit best of all for use in every of EIA modifications were selected. Conditions of the assays were optimized. Concurrent EIAs were 10-20 times less sensitive than sandwich EIA. The developed sandwich EIA permitted ADV assay in preparations of both live and formaldehyde-inactivated virus.
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