Mutants of two strains of Pseudomonas putida expressed two cryptic chloroamidases (C-amidase and H-amidase) and one cryptic dehalogenase (DehII). The mutants were selected on either 2-chloropropionamide (2CPA) or 2-monochloropropionate (2MCPA), developing as papillae in parental colonies growing on a metabolisable support substrate. Mutants expressing C-amidase were selected if 2CPA was utilised as either a carbon or a nitrogen source. H-amidase mutants were selected only if 2CPA was used as a nitrogen source. Growth temperature and pH affected the frequency of papillae production, although different temperatures and pHs did not affect the overall growth characteristics of the parental colonies. Decreasing growth temperature increased the frequency of 2cpa+ papillae formation, but decreased the frequency of 2mcpa+ papillae formation. Low pH (6.0) prevented the formation of 2mcpa+ and 2cpa+ papillae. However, in the case of the 2cpa+ papillae, decreasing the growth temperature also allowed papillae formation at pH 6.0.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF00262204 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Influence of Source of Origin and Region of Finishing on Growth Performance and Carcass Characteristics of Finishing Heifers Fed in the United States.
J Anim Sci
January 2025
Department of Animal Science, South Dakota State University, Brookings, SD, USA.
The objective was to evaluate growth performance and carcass traits of finishing beef heifers sourced and finished in different regions in the U.S. Heifers [n = 190; initial body weight (BW) 483 ± 0.
View Article and Find Full Text PDFModeling the Impact of Seasonality on Mosquito Population Dynamics: Insights for Vector Control Strategies.
Bull Math Biol
January 2025
Department of Biology, Memorial University of Newfoundland, St. John's, NL, Canada.
Mosquitoes are important vectors for the transmission of some major infectious diseases of humans, i.e., malaria, dengue, West Nile Virus and Zika virus.
View Article and Find Full Text PDFDietary and competition effects on life history attributes of Chrysomya megacephala and Lucilia sericata (Diptera: Calliphoridae) in south-west Europe.
Int J Legal Med
January 2025
University of Alicante, Department of Environmental Sciences and Natural Resources, PO Box 99, Alicante, E-03080, Spain.
The range of the oriental latrine fly (Chrysomya megacephala) is currently expanding. It coexists with another blowfly with a similar ecology, the green bottle fly (Lucilia sericata), one of the most abundant species in carrion during warm months. It is essential to understand the influence of temperature, larval substrate type, and larval competition on the development rates of these necrophagous calliphorids to evaluate the role and the adaptation of C.
View Article and Find Full Text PDFCatalyst-derived hierarchy in 2D imine-based covalent organic frameworks.
Nanoscale
January 2025
Dept. of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA 18015, USA.
Identifying facile strategies for hierarchically structuring crystalline porous materials is critical for realizing diffusion length scales suitable for broad applications. Here, we elucidate synthesis-structure-function relations governing how room temperature catalytic conditions can be exploited to tune covalent organic framework (COF) growth and thereby access unique hierarchical morphologies without the need to introduce secondary templates or structure directing molecules. Specifically, we demonstrate how scandium triflate, an efficient catalyst involved in the synthesis of imine-based COFs, can be exploited as an effective growth modifier capable of selectively titrating terminal amines on 2D COF layers to facilitate anisotropic crystal growth.
View Article and Find Full Text PDFBuffer-Less Gallium Nitride High Electron Mobility Heterostructures on Silicon.
Adv Mater
January 2025
Department of Materials Science and Metallurgy, University of Cambridge, Cambridge, CB3 0FS, UK.
Thick metamorphic buffers are considered indispensable for III-V semiconductor heteroepitaxy on large lattice and thermal-expansion mismatched silicon substrates. However, III-nitride buffers in conventional GaN-on-Si high electron mobility transistors (HEMT) impose a substantial thermal resistance, deteriorating device efficiency and lifetime by throttling heat extraction. To circumvent this, a systematic methodology for the direct growth of GaN after the AlN nucleation layer on six-inch silicon substrates is demonstrated using metal-organic vapor phase epitaxy (MOVPE).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!