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Assessment of genetic conservation units of an endangered glacial relict insular species, , based on fine-scale genetic structures of multiple fragmented mountainous populations in Taiwan.
Front Plant Sci
January 2025
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
Insular species are usually endemic and prone to long-term population reduction, low genetic diversity, and inbreeding depression, which results in difficulties in species conservation. The situation is even more challenging for the glacial relict species whose habitats are usually fragmented in the mountainous regions. is an endangered and endemic relict tree species in Taiwan.
View Article and Find Full Text PDFDNA Nanotags for Multiplexed Single-Particle Electron Microscopy and Electron Cryotomography.
JACS Au
January 2025
CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, CAS, Beijing 100190, China.
DNA nanostructures present new opportunities as Nanotags for electron microscopy (EM) imaging, leveraging their high programmability, unique shapes, biomolecule conjugation capability, and stability compatible with standard cryogenic sample preparation protocols. This perspective highlights the potential of DNA Nanotags to enable high-throughput multiplexed EM analysis and facilitate particle identification for cryogenic electron tomography (cryo-ET). Meanwhile, applying Nanotags in live-cell environments requires the efficient cellular uptake of intact structures and successful cytosolic migration.
View Article and Find Full Text PDFIn situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology.
Food Chem X
January 2025
State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
To overcome the time-consuming off-site limitations in conventional pesticide detection, this contribution presents an in situ quantitative analysis detection strategy for pesticides on leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology. Taking fungicide metrafenone as a representative, we initially screened seven commonly used matrices, and identifying α-cyano-4-hydroxycinnamic acid as the most effective one in positive mode. Subsequently, coating the matrix using sublimation spraying method resulted in the highest mass intensity.
View Article and Find Full Text PDFChromosome distribution of four LTR retrotransposons and 18 S rDNA in coffea eugenioides.
Sci Rep
January 2025
Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa. ZIP, 36.570-900, Viçosa - MG, Brazil.
Repetitive sequences are recognized for their roles in plant genome organization and function. Mobile elements are notable repeatome sequences due to their intrinsic mutagenic potential, which is related to the origin of adaptive novelties. Understanding the genomic organization and dynamics of the repeatome is fundamental to enlighten their role in plant genome evolution.
View Article and Find Full Text PDFConservative management of placenta accreta spectrum is associated with improved surgical outcomes compared to cesarean hysterectomy: A systematic review and meta-analysis.
Am J Obstet Gynecol
January 2025
Division of Perinatal Surgery and Maternal Fetal Medicine, Department of Obstetrics & Gynecology, Baylor College of Medicine, Houston, TX. Electronic address:
Objective: To compare maternal and surgical outcomes between patients with placenta accreta spectrum (PAS) who underwent conservative management and those who underwent cesarean hysterectomy (CH).
Data Sources: We performed a systematic search in PubMed, Embase and Web of Science from inception up to June 2, 2024.
Study Eligibility Criteria: Studies comparing clinical outcomes among patients with PAS undergoing conservative management versus CH were included.
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