A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR.

Transgenic Res

Unité de Differentiation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.

Published: March 1995

A very simple and reliable method to extract DNA directly from mouse tail, rabbit ear and blood is described. Tissue was homogenized in a solution of NaI and the DNA was extracted using glass powder. The extracted DNA was obtained in sufficient quantity and purity to allow direct detection of transgene by PCR.

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http://dx.doi.org/10.1007/BF01969417DOI Listing

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