Two clones of the K562 human leukemic cell line were isolated according to hemoglobin (Hb) expression. One clone was expressed less than 5% (K562-L) and the other more than 90% (K562-H). The two clones did not exhibit any difference in cell growth or cell cycle. However, the Hb expression of K562-H cells was reduced by succinylacetone (S.A.). The above results suggested that the difference in the Hb production of K562-L and K562-H cells depended on the heme synthetic activity. On the other hand, glycophorin A was expressed to a greater extent on K562-L cells than on K562-H cells. These findings suggested that heme synthesis and the expression of glycophorin A on K562 cells were not always related. The CD11b and the CD61 were also expressed to a greater extent on K562-L cells than on K562-H cells, but the CD34 was not expressed on these cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1248/bpb.16.548 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Growth-inhibition by hemin in K562 human leukemic cells is related to hemoglobin-producing activity.
Biol Pharm Bull
May 1994
Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.
To determine the mechanism of the growth inhibition associated with the induction of erythroid differentiation in K562 cells by hemin, we used two K562 subclones with different hemoglobin (Hb)-producing activity. Hemin strongly inhibited the growth of K562-L, which had a low Hb-producing activity, but not that of K562-H, which had a high Hb-producing activity. When the cell growth of K562-L was inhibited by hemin, the S phase of the cell cycle decreased and the G2/M phase increased.
View Article and Find Full Text PDFEffect of 12-O-tetradecanoylphorbol-13-acetate on expression of cell surface antigens in two subclones of human leukemia K562 cells.
Biol Pharm Bull
October 1993
Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.
Two subclones (K562-L and -H) were previously isolated from K562 human leukemic cells according to hemoglobin production: K562-L was expressed in less than 5% and K562-H in more than 90% of dianisidine positive cells. 12-O-Tetradecanoylphorbol-13- acetate (TPA) suppressed the expression of the erythrocytic (glycophorin A) and myelocytic (CD11b) antigens in K562-L, but increased the expression of these antigens in K562-H. TPA increased the megakaryocytic (CD61) antigens in both cells.
View Article and Find Full Text PDFCloning and characterization of K562 cells on hemoglobin synthetic activity.
Biol Pharm Bull
June 1993
Department of Microbiology, Tohoku College of Pharmacy, Miyagi, Japan.
Two clones of the K562 human leukemic cell line were isolated according to hemoglobin (Hb) expression. One clone was expressed less than 5% (K562-L) and the other more than 90% (K562-H). The two clones did not exhibit any difference in cell growth or cell cycle.
View Article and Find Full Text PDFInhibition of hemoglobin accumulation by monoclonal antibodies to the human transferrin receptor is reversed by hemin.
Exp Hematol
December 1987
Centro di Studi Biochimici sul Morbo di Cooley, Ferrara, Italy.
Article Synopsis
- The study examines how hemin affects the inhibition of erythroid differentiation in K562 cells, particularly when influenced by a monoclonal antibody targeting the human transferrin receptor.
- In the absence of hemin, the antibody suppresses hemoglobin production triggered by treatments like butyric acid or 5-azacytidine.
- However, when hemin is present, a hemin-resistant K562 cell line can produce hemoglobin even with the antibody present, indicating that hemin may help overcome the inhibitory effects of the antibody on iron uptake and hemoglobin synthesis.
5 Azacytidine ribonucleoside (5 Aza CR), greatly enhances erythroid differentiation of the K562(h) cell line, with a sharp increase of embryonic and fetal globin gene expression. This phenomenon is correlated with the undermethylation of gamma-globin but not of epsilon-globin, as the epsilon-globin gene is already extensively undermethylated before 5AzaCR induction. By contrast no variations in both DNA methylation and expression are observed in 7SL RNA genes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!