Fluorescence in situ hybridization for direct visualization of gram-negative anaerobes in subgingival plaque samples.

FEMS Immunol Med Microbiol

Institut für Medizinische Mikrobiologie und Hygiene, Klinikum der Albert-Ludwigs-Universität, Freiburg, FRG.

Published: March 1993

Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora.

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1574-695X.1993.tb00312.xDOI Listing

Publication Analysis

Top Keywords

fluorescence situ
8
situ hybridization
8
subgingival plaque
8
plaque samples
8
hybridization direct
4
direct visualization
4
visualization gram-negative
4
gram-negative anaerobes
4
anaerobes subgingival
4
samples fluorescent
4

Similar Publications

Background: Closed head injury (CHI) provokes a prominent neuroinflammation that may lead to long-term health consequences. Microglia plays pivotal and complex roles in neuroinflammation-mediated neuronal insult and repair following CHI. We previously reported that induced neural stem cells (iNSCs) can block the effects of CXCL12/CXCR4 signaling on NF-κB activation in activated microglia by CXCR4 overexpression.

View Article and Find Full Text PDF

Tissue microenvironments are extremely complex and heterogeneous. It is challenging to study metabolic interaction between the different cell types in a tissue with the techniques that are currently available. Here we describe a multimodal imaging pipeline that allows cell type identification and nanoscale tracing of stable isotope-labeled compounds.

View Article and Find Full Text PDF

Identification of the molecular characterization and tumor microenvironment of thoracic inflammatory myofibroblastic tumors.

J Formos Med Assoc

January 2025

Department of Internal Medicine, National Taiwan University Cancer Center, Taipei, Taiwan; Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan. Electronic address:

Background: Inflammatory myofibroblastic tumors (IMTs), rare soft tissue neoplasms, are characterized by a blend of myofibroblastic proliferation and inflammatory features. While generally characterized by slow growth, IMTs can exhibit locally aggressive behavior, and in rare instances, metastasize to distant sites. This study elucidated the clinical characteristics, molecular profile, and tumor microenvironment of thoracic IMTs.

View Article and Find Full Text PDF

Single-organelle visualization tracking natural glycosaminoglycans within mitochondria-lysosome crosstalk for inflammatory homeostasis.

Int J Biol Macromol

January 2025

School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China; National Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, Shandong 266237, China. Electronic address:

Glycosaminoglycans (GAGs), as natural products with diverse biological activities, play a significant role in regulating inflammatory homeostasis. Nevertheless, the mechanism underlying their intracellular anti-inflammatory properties remains unclear. Herein, we propose a single-organelle visualization tracking framework, leveraging an advanced fluorescent imaging technology combined with labeling methods to dynamically trace the subcellular regulatory mechanisms of GAGs in eliminating inflammatory markers, such as reactive oxygen species (ROS).

View Article and Find Full Text PDF

Fetal fibroblast heterogeneity defines dermal architecture during human embryonic skin development.

J Invest Dermatol

January 2025

Centre for Gene Therapy and Regenerative Medicine, King's College London, Guy's Hospital, Great Maze Pond, London, UK; Directors' Unit, EMBL, Meyerhofstr. 1, 69117 Heidelberg, Germany. Electronic address: https://twitter.com/fionamarywatt.

To investigate heterogeneity of fibroblasts in human fetal skin, we analysed published single-cell RNA sequencing data (8 and 16 post conception weeks (PCW)) and performed single-molecule fluorescence in situ hybridisation to map their spatial distribution and predicted dynamic interactions. Clustering revealed 8 fibroblast populations with developmental stage-specific abundance changes. Proliferative cells (MKI67+) were present at all stages.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!