An automated micromethod for assessment of the lysozyme activity in the blood serum and saliva using a special program is presented. The test is carried out in 96-well polystyrene plates, this helping to save the reagents and spare the test samples without making the assessment less sensitive. The method intensifies the process of measurement, which is particularly evident in prophylactic screening for the detection of groups at risk of body reactivity reduction, local immunity disorders in children and adults, this latter fact being especially valuable.
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Eur J Trauma Emerg Surg
December 2024
Department of Anesthesiology and Critical Care, Tours University Hospital, 37000, Tours, France.
Rev Paul Pediatr
March 2024
Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Ciudad de Córdoba, Córdoba, Argentina.
Objective: To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes.
Methods: Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p<0.
Biosci Biotechnol Biochem
May 2023
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Staphylococcus lugdunensis produces lugdulysin, a metalloprotease that may contribute to its virulence. This study aimed to evaluate the biochemical aspects of lugdulysin and investigate its effect on Staphylococcus aureus biofilms. The protease was isolated and characterized for its optimal pH and temperature, hydrolysis kinetics, and influence of metal cofactor supplementation.
View Article and Find Full Text PDFVet World
February 2022
Department of Biology, Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life (SNAMOPEQ), Faculty of Sciences Dhar Mehraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco.
Background And Aim: Human mycotic infections are one of the major health problems worldwide. Prolonged use of antimycotic drugs has contributed to the development of resistance in pathogenic fungi. This study was conducted to examine antioxidant and anticandidal activities of Moroccan propolis.
View Article and Find Full Text PDFToxicol In Vitro
June 2021
Laboratoire de Toxicologie Génétique, Institut Pasteur de Lille, 1 rue du Pr. Calmette, Lille Cedex 59019, France; Université de Lille, CHU Lille, Institut Pasteur de Lille, EA4483-IMPECS, France. Electronic address:
Processed meat products are presumptive sources of mutagens and genotoxins for consumers. Several epidemiological studies have reported that these products' high intakes have a positive link with cancer incidence. In Algeria, industrially processed meat products are widely consumed.
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