Eight thrombocytopenic/pathic patients received an intravenous infusion of phospholipids. In vitro bleeding test (Thrombostat 4000), thrombelastography, and resonance thrombography were performed in order to show the hemostatic effect. In none of the cases did phospholipids exert an effect comparable to platelet transfusion. Two patients, brother and sister, had a severe anaphylactoid reaction after the administration of phospholipids.
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http://dx.doi.org/10.1055/s-0032-1313607 | DOI Listing |
Cell Mol Biol Lett
January 2025
Department of Analytical Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-069, Bialystok, Poland.
The skin is a barrier that protects the human body against environmental factors (physical, including solar radiation, chemicals, and pathogens). The integrity and, consequently, the effective metabolic activity of skin cells is ensured by the cell membrane, the important structural and metabolic elements of which are phospholipids. Phospholipids are subject to continuous transformation, including enzymatic hydrolysis (with the participation of phospholipases A, C, and D) to free polyunsaturated fatty acids (PUFAs), which under the influence of cyclooxygenases (COX1/2), lipoxygenases (LOXs), and cytochrome P450 (CYPs P450) are metabolized to various classes of oxylipins, depending on the type of PUFA being metabolized and the enzyme acting.
View Article and Find Full Text PDFAAPS PharmSciTech
January 2025
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
The transdermal route is one of the effective routes for delivering drugs. It also overcomes many limitations associated with oral delivery. One of the limitations of this route is the drug's poor skin permeability-stratum corneum, the skin's outermost layer that also acts as a barrier for the drug to penetrate.
View Article and Find Full Text PDFNat Commun
January 2025
State Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, 510632, China.
Oxidative stress plays a critical role in postmenopausal osteoporosis, yet its impact on osteoblasts remains underexplored, limiting therapeutic advances. Our study identifies phospholipid peroxidation in osteoblasts as a key feature of postmenopausal osteoporosis. Estrogen regulates the transcription of glutathione peroxidase 4 (GPX4), an enzyme crucial for reducing phospholipid peroxides in osteoblasts.
View Article and Find Full Text PDFMethods Cell Biol
January 2025
Department of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States. Electronic address:
The externalization of Phosphatidylserine (PS) from the inner surface of the plasma membrane to the outer surface of the plasma membrane is an emblematic event during apoptosis and serves as a potent "eat-me" signal for the efferocytosis of apoptotic cells. Although less well understood, PS is also externalized on live cells in the tumor microenvironment and on live virus-infected cells whereby it serves as an immune modulatory signal that drives tolerance and immune escape. Given the importance of PS in cancer immunology and immune escape, PS-targeting monoclonal antibodies have been characterized with promising immunotherapeutic potential.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. A. S. Nagar, 160062, Punjab, India. Electronic address:
A standardized polyphenol-enriched fraction (IPHRFPPEF) was formulated into a phospholipid complex (IPHRFPPEF-PC) to enhance oral bioavailability and evaluate stability, toxicity, and in vivo anti-inflammatory activity in Sprague Dawley rats. IPHRFPPEF was prepared from crude extract using XAD-HP7/Diaion-HP20 resin column chromatography and analyzed via HPLC and NMR. Total phenolic and flavonoid contents were quantified, with IPHRFPPEF showing higher values than the crude fraction.
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