Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes (CTL) are considered to be one of major defenses against the activation of EBV infection. We have developed a simple assay for evaluation of EBV-CTL by means of culturing peripheral blood mononuclear cells at the concentration of 2 x 10(6) cells/ml infected by B95-8 EBV, in the presence or absence of cyclosporin A (CSA). No lymphoblastoid cell line was established from ten EBV-seropositive healthy individuals in the absence of CSA. In contrast, cell lines were established from ten EBV-seronegative healthy individuals in the absence of CSA, indicating lack of EBV-CTL in these individuals. Methods described herein are simple and correlate well with EBV-CTL activity when compared to conventional methods, such as outgrowth regression or chromium-51 release assays.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0022-1759(95)00082-l | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ultrafast enzyme-responsive hydrogel for real-time assessment and treatment optimization in infected wounds.
J Nanobiotechnology
January 2025
Department of Laboratory Medicine Center, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Guangzhou Medical University, Qingyuan, Guangdong, China.
Monitoring wound infection and providing appropriate treatment are crucial for achieving favorable outcomes. However, the time-consuming nature of laboratory culture tests may delay timely intervention. To tackle this challenge, a simple yet effective HDG hydrogel, composed of hydrogen peroxide (H₂O₂), dopamine, and GelMA polymer, is developed for the ultrafast detection and treatment of Staphylococcus aureus (SA) infections.
View Article and Find Full Text PDFCross-cultural adaptation of the simple Chinese version of the medication adherence reasons scale in patients undergoing adjuvant endocrine therapy for breast cancer.
BMC Cancer
January 2025
Breast Surgery Department, Hangzhou Institute of Medicine, Zhejiang Cancer Hospital, Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Adjuvant endocrine therapy (AET) is essential for improving survival and reducing mortality and recurrence rates in breast cancer (BrCa) patients. However, the adherence to AET among BrCa patients is poor, and there is no scale to measure adherence to AET or the reasons for non-adherence among BrCa patients in mainland China. The aim of this study was to assess the psychometric properties of the simple Chinese version of the Medication Adherence Reasons (MAR) scale in BrCa patients undergoing AET.
View Article and Find Full Text PDFErythema nodosum, malignant melanoma and non-melanoma skin cancer in relation to inflammatory bowel disease: a Mendelian randomization study.
Sci Rep
January 2025
Department of Colorectal Surgery, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, PR China.
Inflammatory bowel disease (IBD) is a multisystem condition that could affect the cutaneous systems, namely cutaneous extraintestinal manifestations (EIMs). It has been suggested that IBD is associated with erythema nodosum (EN), malignant melanoma (MM) and non-melanoma skin cancer (NMSC). However, the potential causal relationship between IBD and the mentioned above cutaneous EIMs is still unclear.
View Article and Find Full Text PDFRational designing of ZIF-67-derived CoO nanocomposite with hierarchical porous structure and extensive peroxidase mimetic activities for highly sensitive colorimetric detection of nitrite in drinking water.
Mikrochim Acta
January 2025
School of Material Science and Engineering, Henan University of Technology Zhengzhou, Henan, 450001, China.
A simple, fast, and cost-effective colorimetric nitrite (NO) sensor based on ZIF-67-derived CoO nanocomposite (ZCo-2 NC) structure has been developed. The prepared colorimetric sensor (ZCo-2 NC) was employed to sensitively detect NO in drinking water system by the exhibition of promising peroxidase-mimicking nanozyme-like features. The sensor manifest well-determined sensing response with excellent linear and wide range of NO sensitivity (0.
View Article and Find Full Text PDFAdditively manufactured ready-to-use platform using conductive recycled PLA for ketamine sensing.
Mikrochim Acta
January 2025
Federal University of Uberlândia, Chemistry Institute, Uberlândia, MG, 38400-902, Brazil.
The use of 3D-printed electrodes is reported fabricated from in-house conductive filament composed of a mixture of recycled poly (lactic acid) (rPLA), graphite (Gpt), and carbon black (CB) for fast detection of the abused drug ketamine. Firstly, the performance of these electrodes was evaluated in comparison to 3D-printed electrodes produced employing a commercially available conductive filament. After a simple pretreatment step (mechanical polishing), the new 3D-printed electrodes presented better performance than the electrodes produced from commercial filament in relation to peak-to-peak separation of the redox probe [Fe(CN)]/ (130 mV and 759 mV, respectively), charge transfer resistance (R = 1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!