AI Article Synopsis
- The study investigated using 19F-[1H] magnetic double-resonance techniques to enhance the sensitivity of 19F NMR for tracking the antitumor drug 5-fluorouracil (5-FU) and its metabolite alpha-fluoro-beta-alanine (FBAL).
- Researchers found that by saturating proton spins, they achieved a significant increase in fluorine signal intensity, which aligned with theoretical expectations for fluorine and hydrogen coupled systems.
- The technique improved detection capabilities, resulting in better signal clarity and noise reduction during 19F NMR assessments in a patient undergoing 5-FU treatment, aiding in the noninvasive monitoring of FBAL levels in the liver.
Article Abstract
19F-[1H] magnetic double-resonance experiments were performed on model solutions of the antitumor drug 5-fluorouracil (5-FU) and of alpha-fluoro-beta-alanine (FBAL) in order to improve 19F NMR sensitivity for the application in pharmacokinetic studies in vivo. Upon driving the proton spins into saturation, a fluorine signal enhancement (nuclear Overhauser effect) was observed on the order of the theoretical NOE maximum gamma H/2 gamma F = 53% for purely dipolar coupled 19F-1H spin systems in extreme narrowing. The dependence of the effect on proton excitation frequency and temporal parameters was measured and cross-relaxation rate constants of 0.07 s-1 (5-FU) and 0.19 s-1 (FBAL) were determined. Irradiation of the proton spin system by a broad pulse during the 19F signal detection period removed FBAL multiplet splittings completely and narrowed the linewidth of this resonance band by a factor of six. Application of proton presaturation in the 19F NMR examination of a patient undergoing 5-FU chemotherapy enhanced the signal-to-noise ratio of the major 5-FU catabolite FBAL detected noninvasively in the liver.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/jmrb.1995.1116 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In vivo imaging of glycogen in human muscle.
Nat Commun
December 2024
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China.
Probing regional glycogen metabolism in humans non-invasively has been challenging due to a lack of sensitive approaches. Here we studied human muscle glycogen dynamics post-exercise with a spatial resolution of millimeters and temporal resolution of minutes, using relayed nuclear Overhauser effect (glycoNOE) MRI. Data at 5T showed a homogeneous distribution of glycogen in resting muscle, with an average concentration of 99 ± 13 mM.
View Article and Find Full Text PDFAltered Nigral Amide Proton Transfer Imaging Signal Concordant With Motor Asymmetry in Parkinson's Disease: A Multipool CEST MRI Study.
NMR Biomed
February 2025
Department of Radiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.
Asymmetry is a natural characteristic of Parkinson's disease (PD), which can be used to distinguish PD from atypical parkinsonism. Chemical exchange saturation transfer (CEST) has demonstrated value in reflecting the subtle changes related to neuron loss and abnormal protein accumulation in PD but has not been used to investigate asymmetry in PD. This study aimed to examine asymmetrical changes in the mesencephalic nucleus of PD patients with motor asymmetry using four-pool CEST analysis and to explore the relationship between imaging asymmetry and motor asymmetry.
View Article and Find Full Text PDFAltered amino and fatty acids metabolism in Sudanese prostate cancer patients: insights from metabolic analysis.
J Circ Biomark
December 2024
Cancer Genomics, International Centre for Genetic Engineering and Biotechnology, Cape Town - South Africa.
Introduction: Prostate cancer (PCa) management presents a multifaceted clinical challenge, intricately linking oncological considerations with cardiovascular health. Despite the recognized importance of lipid metabolism and hypertension in this interwoven relationship, their involvement in PCa development remains partially understood. This study aimed to explore variations in plasma metabolome among Sudanese PCa patients and their associated comorbidities.
View Article and Find Full Text PDFSolid Effect Dynamic Nuclear Polarization Enhancement of >500 at 9.4 T.
J Phys Chem Lett
December 2024
Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Efficient polarizing agents for dynamic nuclear polarization (DNP) enhanced magic angle spinning (MAS) NMR spectroscopy are of high current interest due to the potential to significantly boost NMR sensitivity. While most efforts have centered on cross-effect (CE) or Overhauser effect (OE) mechanisms, yielding enhancement factors up to ∼300 at 9.4 T, radicals yielding solid effect (SE) DNP have seen less development.
View Article and Find Full Text PDFSupramolecular encapsulation of eugenol with acyclic cucurbit[n]urils: Enhancing water solubility and in vitro antioxidant activity.
J Food Sci
December 2024
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, PR China.
In recent years, consumer demand for healthy foods containing natural food additives has increased. Eugenol (EUG) is an essential oil popular as a natural antiseptic. However, its limited water solubility, high volatility, strong odor, and fragile stability hinder its application and storage in the field of food preservation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!