AI Article Synopsis
- The human growth hormone gene can be transcribed from an alternative promoter located 197 base pairs upstream of the main mRNA cap site, as shown in tissue studies.
- The resultant mRNA is specifically found in the hypophysis (pituitary gland) and placenta, but not in other tissue types, and codes for a 107-residue protein with similarities to hepatic nuclear factor-1alpha.
- This protein, when tagged with a specific peptide for detection, showed immunoreactivity in certain cells of the adenohypophysis and could enhance transcription activity when linked to a yeast transcription factor in pituitary and placental cells.
Article Abstract
Transcription of the human growth hormone gene can start in vitro and in vivo 197 base pairs upstream from the cap site of growth hormone mRNA (Courtois, S. J., Lafontaine, D., and Rousseau, G. G. (1992) J. Biol. Chem. 267, 19736-19743). We have now characterized the mRNA that originates from this optional promoter and have found that it occurs in human hypophysis and placenta but not in 10 other tissues. This mRNA contains an open reading frame for a protein of 107 residues that shares sequence similarity with three domains of hepatic nuclear factor-1alpha. With antibodies directed against a peptide corresponding to the C terminus of this protein, immunoreactive material was detected in a subset of cells of the adenohypophysis. When fused to the DNA-binding domain of the yeast transcription factor GAL4, the protein stimulated transcription from a GAL4-sensitive reporter gene in transiently transfected pituitary and placental cells.
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| http://dx.doi.org/10.1074/jbc.270.33.19205 | DOI Listing |
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