AI Article Synopsis
- The human DNA helicase IV (HDH IV) is a 100-kDa protein that unwinds DNA in the 5' to 3' direction and is identical to the cDNA of nucleolin, a crucial protein for pre-ribosome assembly.
- HDH IV/nucleolin can unwind various types of duplexes, including RNA-RNA, DNA-DNA, and DNA-RNA.
- The unwinding activity of HDH IV/nucleolin is enhanced through phosphorylation by specific kinases, and its C-terminal domain contributes to its helicase function with some ATP-dependent activity.
Article Abstract
The cDNA encoding human DNA helicase IV (HDH IV), a 100-kDa protein which unwinds DNA in the 5' to 3' direction with respect to the bound strand, was cloned and sequenced. It was found to be identical to the human cDNA encoding nucleolin, a ubiquitous eukaryotic protein essential for pre-ribosome assembly. HDH IV/nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Phosphorylation of HDH IV/nucleolin by cdc2 kinase and casein kinase II enhanced its unwinding activity in an additive way. The Gly-rich C-terminal domain possesses a limited ATP-dependent duplex-unwinding activity which contributes to the helicase activity of HDH IV/nucleolin.
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Article Synopsis
- The human DNA helicase IV (HDH IV) is a 100-kDa protein that unwinds DNA in the 5' to 3' direction and is identical to the cDNA of nucleolin, a crucial protein for pre-ribosome assembly.
- HDH IV/nucleolin can unwind various types of duplexes, including RNA-RNA, DNA-DNA, and DNA-RNA.
- The unwinding activity of HDH IV/nucleolin is enhanced through phosphorylation by specific kinases, and its C-terminal domain contributes to its helicase function with some ATP-dependent activity.
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