Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, deletions, insertions) localized in the analyzed amplicon appear to be suitable for molecular-epidemiological studies. A scheme of spreading of the molecular markers in the population is suggested.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0166-0934(95)00002-c | DOI Listing |
Publication Analysis
Top Keywords
molecular markers
8
optimization pcr-based
4
pcr-based diagnostics
4
diagnostics human
4
human cytomegalovirus
4
cytomegalovirus techniques
4
techniques dna
4
dna template
4
template preparation
4
preparation pcr-based
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!