A dramatic shortage of kidneys available for transplantation, due do the widespread reduction in consent, has urged us to explore alternative sources of kidney harvesting, in the attempt to reduce the current gap between the small number of donations and the large number of patients on waiting-list. We set our attention on non-heart-beating donors (NHBD). In the "first step" of our research, we tested an experimental technique, by using commercial pigs' kidneys, that has been useful to determinate the maximum warm ischemic time that a kidney of NHBD could tolerate. Parameters of this research were the resistance of the kidney in perfusion machine and the renal scintigraphy. Our results permitted to establish that the maximum warm ischemic time that a kidney of NHBD could tolerate, is 50 minutes (p > 0.001). This experimental technique could be employed to remove every doubt about the effective condition of NHBD kidney before the transplant, in the outlook, by using those kidneys, to reduce the organ shortage.
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Environ Health Perspect
January 2025
Division of Experimental Medicine, Department of Medicine, McGill University, Montréal, Canada.
Background: Millions worldwide are exposed to elevated levels of arsenic that significantly increase their risk of developing atherosclerosis, a pathology primarily driven by immune cells. While the impact of arsenic on immune cell populations in atherosclerotic plaques has been broadly characterized, cellular heterogeneity is a substantial barrier to in-depth examinations of the cellular dynamics for varying immune cell populations.
Objectives: This study aimed to conduct single-cell multi-omics profiling of atherosclerotic plaques in apolipoprotein E knockout () mice to elucidate transcriptomic and epigenetic changes in immune cells induced by arsenic exposure.
Astrobiology
January 2025
NASA Goddard Space Flight Center, Greenbelt, Maryland, USA.
Meteoritic impacts on planetary surfaces deliver a significant amount of energy that can produce prebiotic organic compounds such as cyanides, which may be a key step to the formation of biomolecules. To study the chemical processes of impact-induced organic synthesis, we simulated the physicochemical processes of hypervelocity impacts (HVI) in experiments with both high-speed C projectiles and laser ablation. In the first approach, a C beam was accelerated to collide with ammonium nitrate (NHNO) to reproduce the shock process and plume generation of meteoritic impacts on nitrogen-rich planetary surfaces.
View Article and Find Full Text PDFPLoS Biol
January 2025
Department of Neurology, School of Medicine and Health, Technical University of Munich (TUM), Munich, Germany.
Pain is closely linked to alpha oscillations (8 < 13 Hz) which are thought to represent a supra-modal, top-down mediated gating mechanism that shapes sensory processing. Consequently, alpha oscillations might also shape the cerebral processing of nociceptive input and eventually the perception of pain. To test this mechanistic hypothesis, we designed a sham-controlled and double-blind electroencephalography (EEG)-based neurofeedback study.
View Article and Find Full Text PDFPLoS Biol
January 2025
Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.
Antibodies are extensively used in biomedical research, clinical fields, and disease treatment. However, to enhance the reproducibility and reliability of antibody-based experiments, it is crucial to have a detailed understanding of the antibody's target specificity and epitope. In this study, we developed a high-throughput and precise epitope analysis method, DECODE (Decoding Epitope Composition by Optimized-mRNA-display, Data analysis, and Expression sequencing).
View Article and Find Full Text PDFPLoS One
January 2025
Computer Engineering, CCSIT, King Faisal University, Al Hufuf, Kingdom of Saudi Arabia.
This paper presents a low-power, second-order composite source-follower-based filter architecture optimized for biomedical signal processing, particularly ECG and EEG applications. Source-follower-based filters are recommended in the literature for high-frequency applications due to their lower power consumption when compared to filters with alternative topologies. However, they are not suitable for biomedical applications requiring low cutoff frequencies as they are designed to operate in the saturation region.
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