The strains of Streptomyces kanamyceticus 1375 and 1 were exposed to N-nitroso-N-methyl urea, ethidium bromide and acriflavine and mutants with impaired biosynthesis of kanamycin (Kan-) were isolated. The majority of the mutants were genetically unstable and segregated with the formation of both the Kan- and Kan+ clones. The stable Kan- mutants were tested in pairs for the cosynthesis. The capacity of the mutants for the recovery of the kanamycin synthesis in media supplemented with kanamycin precursors such as 2-desoxystreptamine and D-glucoseamine was studied. Four classes of the Kan- mutants involving various genes controlling the kanamycin biosynthesis in S. kanamyceticus were identified by the results of the tests.
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