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Trans-nuclei CRISPR/Cas9: safe approach for genome editing in the edible mushroom excluding foreign DNA sequences.
Appl Microbiol Biotechnol
December 2024
Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kitashirakawaoiwakecho, Kyoto, 606-8502, Japan.
Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-assisted genome editing has been applied to several major edible agaricomycetes, enabling efficient gene targeting. This method is promising for rapid and efficient breeding to isolate high-value cultivars and overcome cultivation challenges. However, the integration of foreign DNA fragments during this process raises concerns regarding genetically modified organisms (GMOs) and their regulatory restrictions.
View Article and Find Full Text PDFMicrobiome of the External Auditory Canal: Changes After Long-Term Hearing Aid Use.
Otol Neurotol
July 2024
Department of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital.
Objective: To investigate the microbial changes of long-term hearing aid use culture independently.
Study Design: Cross-sectional study.
Patients: Fifty long-term hearing aid users and 80 volunteer controls with asymptomatic ears.
Mefloquine-curcumin combinations improve host mitochondrial respiration and decrease mitotoxic effects of mefloquine in -infected mice.
Curr Res Pharmacol Drug Discov
April 2024
Laboratories for Biomemebrane Research and Biotechnology, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
infection is a health challenge. Although, antiplasmodial drugs kill the parasites, information on the effects of infection and drugs on the expression of some genes is limited. Malaria was induced in two different studies using NK65 (chloroquine-susceptible, study 1), and ANKA (chloroquine-resistant, study 2) strains of in 30 male Swiss mice (n = 5) in each study.
View Article and Find Full Text PDFValidation of a simplified small-scale DNA extraction protocol from wine by quantitative real-time PCR.
3 Biotech
May 2024
Department of Life Sciences, University of Siena, 53100 Siena, Italy.
Unlabelled: In the present study, we compared a simplified small-scale purification protocol to obtain DNA admixtures out of wine, with our large-scale published method. The extraction methods must provide DNA free of PCR inhibitors, that can interfere with DNA amplification. To evaluate the efficiency of grapevine's nuclear DNA extraction from wine, the new protocol was also compared in terms of purity and yield to the DNA obtained out of grapevine's () leaf tissue, using a commercial kit.
View Article and Find Full Text PDFDevelopment of a Point-of-Care Microfluidic RNA Extraction Slide for Gene Expression Diagnosis after Irradiation.
Radiat Res
May 2024
Bundeswehr Institute of Radiobiology affiliated to the University of Ulm, Neuherbergstraße 11, 80937 Munich, Germany.
In times of war, radiological/nuclear emergency scenarios have become a reemphasized threat. However, there are challenges in transferring whole-blood samples to laboratories for specialized diagnostics using RNA. This project aims to miniaturize the process of unwieldy conventional RNA extraction with its stationed technical equipment using a microfluidic-based slide (MBS) for point-of-care diagnostics.
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