An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC177523 | PMC |
| http://dx.doi.org/10.1128/jb.177.22.6666-6669.1995 | DOI Listing |
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Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.
J Bacteriol
November 1995
Department of Microbiology, University of Groningen, Haren, The Netherlands.
An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E.
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