Purification of a N-acetyl-D-galactosamine specific lectin from the orchid Laelia autumnalis.

Phytochemistry

Departamento de Bioquímica, Facultad de Medicina, UNAM, Mexico DF.

Published: October 1995

From the pseudobulbs of the orchid L. autumnalis a lectin was purified on immobilized porcine mucin with A + H blood group substance. This lectin is a dimeric glycoprotein of M(r) 12,000 with an Sw,20 of 2.2, showing haemagglutinating activity directed mainly to human A1 desialylated erythrocytes. The lectin possesses sugar specificity for N-acetyl-D-galactosamine and also shows high specificity for glycoproteins containing the T (galactose beta 1,3GA1NAc alpha 1,0 Ser/Thr) or the Tn antigen (GalNAc alpha 1,0 Ser/Thr).

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http://dx.doi.org/10.1016/0031-9422(95)00295-iDOI Listing

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