Near-field scanning optical microscopy of phospholipid monolayers doped with fluorescent lipid analogs reveals previously undescribed features in various phases, including a concentration gradient at the liquid-expanded/liquid-condensed domain boundary and weblike structures in the solid-condensed phase. Presumably, the web structures are grain boundaries between crystalline solid lipid. These structures are strongly modulated by the addition of low concentrations of cholesterol and ganglioside GM1 in the monolayer.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1126/science.270.5236.610 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Melittin-Induced Structural Transformations in DMPG and DMPS Lipid Membranes: A Langmuir Monolayer and AFM Study.
Molecules
December 2024
Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, ul. Zwirki i Wigury 101, 02-089 Warsaw, Poland.
In this study, we explore the interactions between melittin, a cationic antimicrobial peptide, and model lipid membranes composed of the negatively charged phospholipids 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG) and 1,2-dimyristoyl-sn-glycero-3-phosphoserine (DMPS). Using the Langmuir monolayer technique and atomic force microscopy (AFM), we reveal novel insights into these interactions. Our key finding is the observation of the ripple phase in the DMPS bilayer on mica, a phenomenon not previously reported for negatively charged single bilayers.
View Article and Find Full Text PDFDipole Potential of Monolayers with Biologically Relevant Lipid Compositions.
Molecules
December 2024
Coimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, Portugal.
The membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes is very relevant. In this work, the dipole potentials of monolayers, formed at the air-water interface, from pure or mixed lipids (1-palmitoyl-2-oleoyl--glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl--glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl--glycero-3-phosphatidyserine (POPS), sphingomyelin (SpM) and cholesterol) were measured and correlated with the mean area per lipid.
View Article and Find Full Text PDFWhey-Derived Antimicrobial Anionic Peptide Interaction with Model Membranes and Cells.
Langmuir
January 2025
Departamento de Química, Catedra de Química Biológica, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba 5000, Argentina.
The present work focuses on one of the possible target mechanisms of action of the anionic antimicrobial peptide β-lg derived from trypsin hydrolysis of β-lactoglobulin. After confirmation of bactericidal activity against a pathogenic Gram(+) strain and demonstration of the innocuousness on a eukaryotic cell line, we investigated the interaction of β-lg with monolayers and bilayers of dpPC and dpPC:dpPG as model membranes of eukaryotic and bacterial membranes, respectively. In monolayers, compared to zwitterionic dpPC, in the negatively charged dpPC-dpPG, β-lg injected into the subphase penetrated up to higher surface pressures and showed greater extents of penetration with increasing concentration in the subphase.
View Article and Find Full Text PDFAssembly of graphene oxide reduced graphene oxide in a phospholipid monolayer at air-water interfaces.
Phys Chem Chem Phys
January 2025
Department of Physics, School of Natural Sciences, Shiv Nadar Institution of Eminence, NH91, Tehsil Dadri, G. B. Nagar, Uttar Pradesh 201314, India.
Graphene and its derivatives, such as graphene oxide (GO) and reduced graphene oxide (rGO), have propelled advancements in biosensor research owing to their unique physicochemical and electronic characteristics. To ensure their safe and effective utilization in biological environments, it is crucial to understand how these graphene-based nanomaterials (GNMs) interact with a biological milieu. The present study depicts GNM-induced structural changes in a self-assembled phospholipid monolayer formed at an air-water interface that can be considered to represent one of the leaflets of a cellular membrane.
View Article and Find Full Text PDFAntimicrobial Polymers at the Membrane Interface: Impact of Macromolecular Architecture.
Small
December 2024
Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476, Potsdam, Germany.
Antimicrobial resistance (AMR) is a major cause of death worldwide. This urges the search for alternatives to antibiotics, and antimicrobial polymers hold promise due to their reduced susceptibility to AMR. The topology of such macromolecules has a strong impact on their activity, with bottlebrush architectures outperforming their linear counterparts significantly.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!