AI Article Synopsis
- A high-capacity system was created for monitoring the expression of numerous genes simultaneously using microarrays printed on glass.
- These microarrays allow for the detection of rare gene transcripts with just 2 microliters of hybridization volume, utilizing only 2 micrograms of total messenger RNA.
- The system was used to measure the differential expression of 45 Arabidopsis genes through a method called two-color fluorescence hybridization.
Article Abstract
A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.
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| http://dx.doi.org/10.1126/science.270.5235.467 | DOI Listing |
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