AI Article Synopsis
- Researchers created a modified Salmonella dublin strain that can secrete a hybrid cytolysin using E. coli's secretion system.
- This hybrid cytolysin combines components from Listeria monocytogenes and E. coli's hemolysin, enhancing its function.
- When J774 macrophage cells engulf this modified strain, the cytolysin partially escapes into the cytoplasm, unlike a nonhemolytic control strain that stays contained.
Article Abstract
We describe the construction of an attenuated Salmonella dublin aroA strain which secretes via the Escherichia coli hemolysin secretion machinery an active hybrid cytolysin consisting of listeriolysin from Listeria monocytogenes and the C-terminal secretion signal of E. coli hemolysin. This hemolytic S. dublin strain is partially released into the cytoplasm of the host cell following uptake by J774 macrophage cells, whereas the nonhemolytic control S. dublin aroA strain remains in the phagosome.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC173596 | PMC |
| http://dx.doi.org/10.1128/iai.63.10.4202-4205.1995 | DOI Listing |
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