In gingival pockets of patients with periodontal disease, polymorphonuclear leukocytes (PMN) are in contact with a peculiar exudate, the gingival crevicular fluid (GCF). Because of the pivotal role played by PMN in periodontal disease, we evaluated the ability of GCF in modulating normal human PMN. GCF was obtained from two gingival sites with severe periodontitis (SP) and two gingival sites with only mild periodontitis (MP) in 12 patients. Purified PMN were exposed to GCF from SP and MP sites and, as a control, to sterile culture medium. GCF activity was evaluated by monitoring the modulation of membrane molecules relevant to cell function. Compared to control medium, GCF from SP and MP sites was able to induce an activation status in PMN evidenced by an increased CD11b (62 +/- 9% and 28 +/- 7%, respectively) and f-Met-Leu-Phe (56 +/- 5% and 31 +/- 7%, respectively) receptor expression, with a concomitant reduction of CD62L expression (56 +/- 8% and 23 +/- 7%, respectively). Thus, reflecting the clinical status, GCF from SP sites was significantly more efficient in affecting PMN than GCF from MP sites. Cell size modifications, evaluated as an additional indicator of PMN activation, were consistent with membrane molecule modulation. The difference in PMN-activating capacity between SP and MP was abrogated by the successful completion of an appropriate periodontal therapy that dramatically improved clinical status. This is the first direct demonstration that GCF from periodontitis has the capacity to activate normal resting PMN and that this capacity reflects the magnitude of the inflammatory process that takes place in the gingiva.
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http://dx.doi.org/10.1007/BF01534581 | DOI Listing |
Medicina (Kaunas)
December 2024
Faculty of Dental Medicine, "Dunarea de Jos" University, Al. I. Cuza Street 35, 800216 Galati, Romania.
: This study aimed to evaluate the role of A-PRF (advanced platelet-rich fibrin) in the enhancement of wound healing and protecting the periodontal health of mandibular second molars after the extraction of mandibular third molars. Additionally, the study assessed the levels of pro-inflammatory cytokines in the gingival crevicular fluid (GCF) of mandibular second molars as markers of inflammation. : Twenty-five systemically healthy adult patients with bilateral removal of impacted mandibular third molars were included.
View Article and Find Full Text PDFInt J Dent Hyg
December 2024
Department of Periodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Objective: To evaluate the efficacy of subgingival erythritol powder air polishing (EPAP) for inducing initial healing of shallow-to-moderate periodontal pockets and its effect on gingival crevicular fluid (GCF) β-catenin, 8-hydroxy-deoxyguanosine (8-OHdG) and total antioxidant capacity (TAC) levels.
Methods: This randomised, split-mouth clinical trial involved 22 periodontitis patients. Periodontal pockets (4-6 mm) were treated either by scaling and root planing (control) or EPAP followed by subgingival instrumentation 1 month later (test).
Clin Oral Investig
December 2024
Department of Periodontology, Faculty of Dentistry, İstinye University, İstanbul, Turkey.
Objectives: Activin-A belongs to the transforming growth factor-beta superfamily and is a multifunctional cytokine that plays a role in inflammation, immune response, tissue repair and regeneration. Proinflammatory cytokine interleukin-1beta (IL-1β) can increase Activin-A expression in various cell types. This study aims to evaluate gingival crevicular fluid (GCF) and salivary Activin-A and IL-β levels in stage III periodontitis.
View Article and Find Full Text PDFJ Clin Periodontol
November 2024
Department of Conservative Dentistry and Periodontology, LMU University Hospital, LMU Munich, Munich, Germany.
Aim: To analyse whether some selected inflammatory biomarkers collected from venous blood and gingival crevicular fluid (GCF) were associated with the outcome of non-surgical periodontal therapy.
Materials And Methods: Two-hundred and nine patients affected by periodontitis were enrolled in the study, who had undergone steps I and II therapy as well as a non-surgical re-instrumentation (NSRI) of periodontal pockets after 6 months. Serum (SE), plasma (PL) and GCF samples were quantitatively analysed for the following inflammatory biomarkers: active matrix metalloproteinase-8 (aMMP-8), prostaglandin E2 (PGE2) and surfactant protein D (SP-D).
Br J Sports Med
December 2024
Orthopedic Surgery, Twin Cities Orthopedics Edina-Crosstown, Edina, Minnesota, USA
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