Effects of orochemical stimulation on postnatal development of gustatory recipient zones within the nucleus of the solitary tract.

Previous receptor damage studies and artificial rearing (AR) studies in rat have demonstrated that orochemical stimulation between the postnatal ages of P2 and P14 is necessary for development of primary gustatory axons and terminal endings in the rostral nucleus of the solitary tract (NST). Objectives of the present experiment were to evaluate the qualitative nature of orochemical stimulation and amount of orochemical stimulation that is necessary to produce normal axonal and terminal development in gustatory recipient zones of the rostral and intermediate NST. To this end, ultra-pure water, 30, 150, and 500 mM NaCl, 80 and 340 mM lactose, whole rat milk, and rat milk that had been subject to extensive dialysis (12-14 kD MWCO) was delivered to independent groups of rat pups during AR procedures. Unstimulated AR animals and matched mother-reared (MR) animals were used as controls. AR animals received experimental orochemical stimulation between the ages of P4 and P10, and were thereafter returned to lactating dams until the time of weaning; MR animals received experimental orochemical stimulation during the course of normal nursing. Following maturation, anterograde fluorescent dual-labeling experiments were conducted to map the course and distribution of primary gustatory axons within the NST. Results show that experimental stimualtion with water during AR procedures is not sufficient to produce normal development of primary gustatory axons and terminal endings in the gustatory NST. Stimulation with 30, 150, and 500 mM NaCl produced normal axonal development in the NST, as did 80 and 340 mM lactose, whole rat milk, and dialyzed rat milk.(ABSTRACT TRUNCATED AT 250 WORDS)